Bio-sequestration of CO2 Using Carbonic Anhydrase in situ Encapsulated Inside Electrospun Hollow Fibers†

doi:10.7503/cjcu20140059

Abstract

Abstract:

Carbonic anhydrase catalyzed bio-sequestration of CO₂to form HC $O 3 -$ , followed by trapping as solid CaCO₃ is one of the most promising technologies for CO₂ capturing. The effects of reaction condition on the CO₂ hydration using free carbonic anhydrase were systematically investigated. In order to improve the stability of the enzyme and facility its recycling, the carbonic anhydrase was in situ encapsulated inside hollow fibers via a novel co-axial electrospinning technology. Compared with the free enzyme, the immobilized carbonic anhydrase showed much improved thermal stability and suffered much reduced inhibitory effects from cation ions, such as Cu²⁺ and Fe³⁺. After 11 reuses, the immobilized enzyme retained about 81.9% of its original activity by comparing the amount of formed CaCO₃ precipitation. In the presence of immobilized carbonic anhydrase, both calcite and vaterite CaCO₃ solid were formed; while in the absence of enzyme or with free carbonic anhydrase, only calcite CaCO₃ was observed.

Key words: Carbonic anhydrase, CO2 Sequestration, Hollow fiber, Electrospinning, CaCO3

CLC Number:

O643.3

TrendMD:

CUI Jiandong, LI Ying, JI Xiaoyuan, BIAN Hongjie, ZHANG Yufei, SU Zhiguo, MA Guanghui, ZHANG Songping. Bio-sequestration of CO₂ Using Carbonic Anhydrase in situ Encapsulated Inside Electrospun Hollow Fibers^†[J]. Chem. J. Chinese Universities, 2014, 35(9): 1999.

Figures/Tables 8

Fig.1 Schematic illustrations of the setup for co-axial electrospinning and the bio-sequestration of CO2 with carbonic anhydrase(CA) encapsulated within hollow fibers

Fig.2 Effects of CaCl2 solution concentration(A), pH(B), amout of CA(C) and reaction time(D) on the mass of formed CaCO3 precipitation

Fig.3 SEM images of top view(A) and cross-sectional view(B) and CLSM image(C) of hollow fiber with in situ encapsulated FTIC-labeled carbonic anhydrase

Fig.4 Thermal stability of the free(a) and immobilized(b) carbonic anhydrase(CA)

Table 1 Effect of metal ions on carbonic anhydrase activity

Metal ion(1 mmol/L)	Relative activity(%)		Metal ion(1 mmol/L)	Relative activity(%)
Metal ion(1 mmol/L)	Free CA		Immobilized CA	Free CA	Immobilized CA
Control	100	100	Mg²⁺	82.51±0.16	80.68±0.33
K⁺	82.16±0.32	91.49±0.28	Zn²⁺	116.13±0.22	107.90±0.42
Li⁺	81.05±0.11	95.99±0.21	Cu²⁺	11.52±0.28	89.07±0.22
Ca²⁺	87.39±0.12	96.84±0.45	Fe³⁺	15.97±0.34	80.38±0.56

Fig.5 Bio-sequestration of CO2

Fig.6 Repeated use of immobilized CA for CO2 bio-sequestration

Fig.7 SEM images of CaCO3 precipitation

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Bio-sequestration of CO₂ Using Carbonic Anhydrase in situ Encapsulated Inside Electrospun Hollow Fibers^†

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