Modification of Baculovirus with Folic Acid based on Phospholipids Self-insertion in Host Cells†

doi:10.7503/cjcu20150886

Abstract

Abstract:

Baculovirus has the advantages such as outstanding biosafety, large cloning capacity and non-replication in the transduced cells. However, their in vivo applications are hampered for lacking of specificity. Therefore, modification of baculovirus with targeting molecules is urgently needed. Herein, a method is proposed for the modification of folic acid(FA) on baculovirus based on phospho-lipids self-insertionin host cells. Immunofluorescent colocalization and transmission electron microscope(TEM) results showed that the modified baculovirus kept their integrity while maintaining their infectivity. Thus the method offers opportunities for the development of tumor-targeting gene vectors and vaccines.

Key words: Baculovirus, Folic acid, Modification, Lipid, Host cell

CLC Number:

O652

TrendMD:

WANG Liying, WEN Li, LÜ Cheng, LIN Yi, PANG Daiwen. Modification of Baculovirus with Folic Acid based on Phospholipids Self-insertion in Host Cells^†[J]. Chem. J. Chinese Universities, 2016, 37(2): 221.

Figures/Tables 5

Fig.1 Bright field images(A, D), fluorescence images(B, E) and their merge(C, F) of Dylight 488 immunofluorescence labeled Sf9 cells incubated without(A—C) and with(D—F) DSPE-PEG2000-FA modification

Fig.2 MTT assay for the toxicity of different concentrations of DSPE-PEG2000-FA incubated with Sf9 cells

Scheme 1 Schematic illustration of the modification of baculovirus with folic acid(FA) assisted by host Sf9 cells incubated with FA functionalized lipids

Fig.3 Bright field images(A, D), fluorescence images of GFP(green) labeled and dylight 649(red) inmmunofluorescence labeled FA-baculovirus(B, E) and their merge on glass coverslips(C, F)

Fig.4 TEM images of BV(A) and FA-BV(B)

References 11

[1]	Chen Y. R., Zhong S. L., Fei Z. J., Hashimoto Y., Xiang J. Z., Zhang S. Y., Blissard G. W., J. Virol., 2013, 87, 6391—6405
[2]	Lin C. Y., Lu C. H., Luo W. Y., Chang Y.H., Sung L. Y., Chiu H. Y., Hu Y. C., Curr. Gene Ther., 2010, 10, 242—254
[3]	Kim Y. K., Park I. K., Jiang H. L., Choi J. Y., Je Y. H., Jin H., Kim H. W., Cho M. H., Cho C. S., J. Biotechnol., 2006, 125, 104—109
[4]	Makela A. R., Enback J., Laakkonen J. P., Vihinen-Ranta M., Laakkonen P., Oker-Blom C., J. Gene. Med., 2008, 10, 1019—1031
[5]	Yang Y., Lo S. L., Yang J. Y., Yang J., Goh S. S. L., Wu C. X., Feng S. S., Wang S., Biomaterials, 2009, 30, 5767—5774
[6]	Kamen B. A., Capdevila A., Proc. Natl. Acad. Sci. USA, 1986, 83, 5983—5987
[7]	Kim Y. K., Choi J. Y., Yoo M. K., Jiang H. L., Arote R., Je Y. H., Cho M. H., Cho C. S., J. Biotechnol., 2007, 131, 353—361
[8]	Banerjee P. S., Ostapchuk P., Hearing P., Carrico I., J. Am. Chem. Soc., 2010, 132, 13615—13617
[9]	Zeng Q. B., Wen H. B., Wen Q., Chen X. H., Wang Y. G., Xuan W. L., Liang J. S., Wan S. H., Biomaterials, 2013, 34, 4632—4642
[10]	Huang B. H., Lin Y., Zhang Z. L., Zhuan F. F., Liu A. A., Xie M., Tian Z. Q., Zhang Z. F., Wang H. Z., Pang D. W., ACS Chem. Biol., 2012, 7, 683—688
[11]	Chen G., Zhu J. Y., Zhang Z. L., Zhang W., Ren J. G., Wu M., Hong Z. Y., Lv C., Pang D. W., Zhao Y. F., Angew. Chem. In. Ed., 2015, 54, 1036—1040

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