Study of Interaction Between Bovine Serum Albumin and Cobalt Complexes of Phenanthroline and Nitrilotriacetate by Fluorescence Spectrometry

doi:10.7503/cjcu20130098

Abstract

Abstract:

Cobalt complexes [Co(phen)₂Cl₂(1), Na[Co(nta)]·H₂O(2), [Co(phen)₂(H₂O)₂]·[Co(nta)(phen)]₂·12H₂O([STHZ]3[STBZ])] (phen: phenanthroline, nta: nitrilotriacetate) were synthesized in solution, and the structure of complex 3 was characterized by single-crystal X-ray diffraction analysis(XRD). It crystallized in the triclinic system, space group P1 with a=1.2448(2) nm, b=1.5898(3) nm, c=1.7412(3) nm, α=91.746(3)°, β=97.807(3)°, γ=103.745(3)°, V=3.309(1) nm³. The interaction between bovine serum albumin(BSA) and complexes 1-3 was investigated with fluorescence spectra at room temperature. Fluorescence quenchings were shown as static quenching for complexes 1 and 3 based on the fluorescence spectrum at different temperatures. The binding constant, binding sites and thermodynamic functions were calculated. The interaction sites, binding force and distance for BSA with complexes 1 and 3 were discussed respectively.

Key words: Cobalt(Ⅱ), Phenanthroline, Nitrilotriacetic acid, Bovine serum albumin(BSA), Fluorescence spectrum

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LIN Hai-Bin, ZHENG Lin, LIN Yu-Qin, ZHOU Zhao-Hui. Study of Interaction Between Bovine Serum Albumin and Cobalt Complexes of Phenanthroline and Nitrilotriacetate by Fluorescence Spectrometry[J]. Chem. J. Chinese Universities, 2013, 34(8): 1818.

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Study of Interaction Between Bovine Serum Albumin and Cobalt Complexes of Phenanthroline and Nitrilotriacetate by Fluorescence Spectrometry

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