Chem. J. Chinese Universities ›› 2017, Vol. 38 ›› Issue (7): 1140.doi: 10.7503/cjcu20170012
• Analytical Chemistry • Previous Articles Next Articles
YUAN Haojun1,2, GAO Wanlei1,2, JING Fengxiang1, LIU Songsheng1, ZHOU Hongbo1, JIA Chunping1,*(), JIN Qinghui1, ZHAO Jianlong1
Received:
2017-01-09
Online:
2017-07-10
Published:
2017-06-12
Contact:
JIA Chunping
E-mail:jiachp@mail.sim.ac.cn
Supported by:
CLC Number:
TrendMD:
YUAN Haojun, GAO Wanlei, JING Fengxiang, LIU Songsheng, ZHOU Hongbo, JIA Chunping, JIN Qinghui, ZHAO Jianlong. Novel Microfluidic Droplet Digital PCR Chip for High Sensitive Detection of Nucleic Acid†[J]. Chem. J. Chinese Universities, 2017, 38(7): 1140.
Fig.1 Design and operation of ddPCR device(A) Schematic representation of the ddPCR device; (B) structure of the PDMS chip(inset shows the generation of droplets under the microscope); (C) an image of the fabricated ddPCR chip.
Sample | Sequence(5'—3') |
---|---|
Forward primer | atcccagaaggtgagaaagt |
Reverse primer | tgtggagatgagcagggtct |
MGB probe | HEX-aagccaacaaggaaatc-MGB; FAM-agatgttttgatagcgac-MGB |
Table 1 DNA sequences of primers and MGB probe
Sample | Sequence(5'—3') |
---|---|
Forward primer | atcccagaaggtgagaaagt |
Reverse primer | tgtggagatgagcagggtct |
MGB probe | HEX-aagccaacaaggaaatc-MGB; FAM-agatgttttgatagcgac-MGB |
Fig.6 Detection of the EGFR exon19 rare mutant(0—0.01%) in the presence of wild type DNA by droplet digital PCR (A1—A4) Hex channel; (B1—B4) Fam channel. Rare mutant: (A1, B1) 0%; (A2, B2) 0.0001%; (A3, B3) 0.001%; (A4, B4) 0.01%.
Fig.7 Detection of the EGFR exon19 rare mutant(0.1%—100%)in the presence of wild type DNA by droplet digital PCR (A1—A4) Hex channel; (B1—B4) Fam channel. Rare mutant: (A1, B1) 0.1%; (A2, B2) 1%; (A3, B3) 10%; (A4, B4) 100%.
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