Determination of Sequence-specific DNA Related to the Avian Influenza H1N1 Virus by Fluorescent Probe Ru(phen)2(dppx)2+

Chem. J. Chinese Universities ›› 2011, Vol. 32 ›› Issue (8): 1737.

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Determination of Sequence-specific DNA Related to the Avian Influenza H1N1 Virus by Fluorescent Probe Ru(phen)2(dppx)2+

ZENG Guo-Ping^1,2, XIANG Dong-Shan², CAI Jin-Zhang², HE Zhi-Ke^2*

1. College of Chemistry and Biology Science, Hubei University of Education, Wuhan 430205, China;
2. Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China

Received:2010-09-27 Revised:2011-04-16 Online:2011-08-10 Published:2011-07-19
Contact: HE Zhi-Ke E-mail:zhkhe@whu.edu.cn
Supported by:
国家自然科学基金(批准号: 21075093, 20921062)资助.

Abstract

Abstract: The determination of sequence-specific DNA related to the avian influenza H1N1 virus was realized with the molecular 'Light Switch' complex of Ru(phen)2dppx2+. There is no or weak fluorescence between the Fluorescent Probe Ru(phen)2dppx2+ and ssDNA probe. However, the luminescent enhancement was observed in presence of target DNA resulted from the intercalation of the complex into the hybrids between probe DNA and target DNA. Under the optimum conditions, the linear range of the DNA related to the avian influenza H1N1 virus is 9.3×10-10～7.4×10-8 mol/L, the calibration curve is y = 3.3829x + 8.3948, R2 =0.9982, and the detection limit is 5.3×10-10 mol/L. This method is simple, rapid, sensitive and selective.

Key words: Fluorescent probe Ru(phen)2(dppx)2+, Avian influenza H1N1 virus, Fluorescence spectrum, Quantitative detection

CLC Number:

中图分类号：O657

TrendMD:

ZENG Guo-Ping, XIANG Dong-Shan, CAI Jin-Zhang, HE Zhi-Ke*. Determination of Sequence-specific DNA Related to the Avian Influenza H1N1 Virus by Fluorescent Probe Ru(phen)2(dppx)2+[J]. Chem. J. Chinese Universities, 2011, 32(8): 1737.

References

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Determination of Sequence-specific DNA Related to the Avian Influenza H1N1 Virus by Fluorescent Probe Ru(phen)2(dppx)2+

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