Immobilization of Angiotensin Converting-enzyme on Chitosan Microspheres

Chem. J. Chinese Universities

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Immobilization of Angiotensin Converting-enzyme on Chitosan Microspheres

HONG Yun-Jia, LI Tan-Yao, CHEN Bo^*, YAO Shou-Zhuo

Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, Changsha 410081, China

Received:2008-02-28 Revised:1900-01-01 Online:2009-02-10 Published:2009-02-10
Contact: CHEN Bo

Abstract

Abstract: Chitosan is a polysaccharide which is widely used in enzyme immobilization as a support. Angiotensin converting-enzyme(ACE) is an important member of the rennin-angiotensin-aldosteronesystem which could cause high blood pressure. In this study, ACE was immobilized on chitosan microspheres with glutaraldehyde as cross-linking reagent. The optimum conditions and properties of immobilization enzyme were investigated. The max ratio activity was 0.085 U/g under 2.5% glutaraldehyde and 8 mg/mL soluble enzyme for immobilized enzyme. The optimum pH, temperature and K_m of the immobilized enzyme were 7—9, 40—50 ℃ and 2.39 mmol/L. The immobilized ACE show higher thermal stability and can be used repeatedly to a certain extent. It was a fundamental study in order to develop the way of screening ACE inhibitor.

Key words: Chitosan, Angiotensin converting-enzyme, Immobilized enzyme

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HONG Yun-Jia, LI Tan-Yao, CHEN Bo^*, YAO Shou-Zhuo. Immobilization of Angiotensin Converting-enzyme on Chitosan Microspheres[J]. Chem. J. Chinese Universities, doi: .

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Immobilization of Angiotensin Converting-enzyme on Chitosan Microspheres

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