Fast Determination of Protein Diffusion Coefficient by Taylor Dispersion Analysis and Capillary Electrophoresis System

Chem. J. Chinese Universities

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Fast Determination of Protein Diffusion Coefficient by Taylor Dispersion Analysis and Capillary Electrophoresis System

ZUO Min^1,2, CHEN Yi¹*

1. Laboratory of Analytical Chemistry for Life Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China;
2. Graduate School, Chinese Academy of Sciences, Beijing 100039, China

Received:2007-05-21 Revised:1900-01-01 Online:2007-10-10 Published:2007-10-10
Contact: CHEN Yi

Abstract

Abstract: A method for the fast and accurate measurement of diffusion coefficient of minute proteins was developed. A protein sample plug was introduced into the capillary and forced to flow through a capillary coated dynamically by poly(diallyldimethylammonium chloride). Its concentration profile was then monitored by the UV absorption detector mounted on Beckman MDQ capillary electrophoresis system. Accurate diffusion coefficient was obtained. The key conditions for the accurate measurement include: (1) systematic peak should be developed in a laminar flow; (2) radius diffusion is negligible compared with the longitudinal diffusion; (3) the capillary is well thermostated and (4) the solute should not be overloaded. In common case, the deviation of the measurement was kept within 4%.

Key words: Minute protein, Diffusion coefficient, Capillary electrophoresis, Taylor analysis

CLC Number:

O657

TrendMD:

ZUO Min^1,2, CHEN Yi¹*. Fast Determination of Protein Diffusion Coefficient by Taylor Dispersion Analysis and Capillary Electrophoresis System[J]. Chem. J. Chinese Universities, doi: .

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Fast Determination of Protein Diffusion Coefficient by Taylor Dispersion Analysis and Capillary Electrophoresis System

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