Determination of Escherichia Coli. O157:H7 in Food Based on Chemiluminescent Magnetic Enzyme-linked Immunoassay

Chem. J. Chinese Universities

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Determination of Escherichia Coli. O157:H7 in Food Based on Chemiluminescent Magnetic Enzyme-linked Immunoassay

ZHU Guang-Hua¹, HE Yan-Feng¹, GUO Xiao-Ying², LIU He-Ping³

1. College of Public Health, Southeast University, Nanjing 210009, China;
2. State Key Laboratory for Molecular Electronics, Department of Biological Science and Medicinal Engineering, Southeast University, Nanjing 210096, China;
3. Jiangsu Centers for Disease Prevention and Control, Naijing 210009, China

Received:2005-12-22 Revised:1900-01-01 Online:2006-08-10 Published:2006-08-10
Contact: ZHU Guang-Hua

Abstract

Abstract: A high sensitive chemiluminescent magnetic enzyme-linked immunoassay method was established for Escherichia coli. O157:H7 determination. The bacterium antibody was labeled by alkaline phosphatase(ALP) that catalyzed the decomposing of substrate 3-(2-spiroadamantane)-4-methoxy-4(3-phosphoryloxy)phenyl-1,2-dioxetane(AMPPD) to give the light emission. The sensitivity of the method is 8.5×10⁴Cell/mL with a linear range of 1.0×10⁵—5.0×10⁷ Cell/mL. The intra- and inter-assay CVs are 14.8% and 20.0% in pork samples, respectively. The correlation coefficient of present and the standard plate counting method is 0.981. The experiments with the spiked samples show that this method has great potential to be applied to detecting the concentration of Escherichia coli. O157:H7 in a variety of samples.

Key words: Immunoassay, Chemiluminescence, Magnetic beads, Escherichia coli.

CLC Number:

O657.39

TrendMD:

ZHU Guang-Hua¹, HE Yan-Feng¹, GUO Xiao-Ying², LIU He-Ping³. Determination of Escherichia Coli. O157:H7 in Food Based on Chemiluminescent Magnetic Enzyme-linked Immunoassay[J]. Chem. J. Chinese Universities, doi: .

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Determination of Escherichia Coli. O157:H7 in Food Based on Chemiluminescent Magnetic Enzyme-linked Immunoassay

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