Rapid Screening of Lipase Inhibitors from Fructus aurantii Using Lipase-immobilized Magnetic Beads Coupled with High-performance Liquid Chromatography-Mass Spectrometry

doi:10.7503/cjcu20120436S

Chem. J. Chinese Universities ›› 2012, Vol. 33 ›› Issue (12): 2692.doi: 10.7503/cjcu20120436S

• Organic Chemistry • Previous Articles Next Articles

Rapid Screening of Lipase Inhibitors from Fructus aurantii Using Lipase-immobilized Magnetic Beads Coupled with High-performance Liquid Chromatography-Mass Spectrometry

CHEN Zhui¹, TAO Yi¹, WANG Yi², WANG Yi¹

1. Pharmaceutical Informatics Institute, Zhejiang University, Hangzhou 310058, China;
2. Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China

Received:2012-05-04 Online:2012-12-10 Published:2012-11-20

Abstract

Abstract:

A new method for the rapidly screening of lipase inhibitors from extracts of traditional Chinese medicine using lipase-immobilized magnetic beads coupled with high-performance liquid chromatography-mass spectrometry(LC-MS) was proposed. After the lipase-immobilized magnetic beads were prepared, they were incubated with total flavonoid of Fructus aurantii to fish the ligands of lipase. Then, the eluent of magnetic beads' screening was analyzed by LC-MS. Four compounds, as naringin, hesperidin, neohesperidin and poncirin, were identified as the potential lipase inhibitors. Furthermore, the in vitro inhibitory activities against lipase of four compounds were tested. The results showed that hesperidin, neohesperidin and poncirin exhibit lipase inhibitory activities, while poncirin was firstly reported to be a lipase inhibitor. Our research showed that active compounds could be rapidly screened from extracts of traditional Chinese medicine via lipase-immobilized magnetic beads coupled with LC-MS.

Key words: Lipase, Magnetic beads' screening, Fructus aurantii, Ligand fishing

CLC Number:

O629

TrendMD:

CHEN Zhui, TAO Yi, WANG Yi, WANG Yi. Rapid Screening of Lipase Inhibitors from Fructus aurantii Using Lipase-immobilized Magnetic Beads Coupled with High-performance Liquid Chromatography-Mass Spectrometry[J]. Chem. J. Chinese Universities, 2012, 33(12): 2692.

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Rapid Screening of Lipase Inhibitors from Fructus aurantii Using Lipase-immobilized Magnetic Beads Coupled with High-performance Liquid Chromatography-Mass Spectrometry

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