Biomolecule-assisted Surface-enhanced Raman Scattering(SERS) Technology and SERS Biosensing†

doi:10.7503/cjcu20150744

Abstract

Abstract:

Biomolecule-assisted surface-enhanced Raman scattering(SERS) sensing is a technique using biological molecules as sensitive components or detected objects, which is one of the most important tools in the studies of biological molecules. SERS biosensing is widely used in environmental monitoring, food-safety, clinical testing, disease diagnosis, and many other areas. This paper summarizes the progresses on the application of SERS technique in the fields of the analysis and diagnosis of cancer. The contents mainly consist of three parts: (1) SERS biosensing technology based the new coupling mechanisms, involving SPR-coupling, waveguide-coupling and LSPR-PSPR coenhancement; (2) preparation and application of highly sensitive SERS chips; and (3) new SERS technology for investigating cancer cells and bio tissues.

Key words: Surface-enhanced Raman scattering, Biosensor, SERS chip, Immunoassay, Cancer

CLC Number:

O657.37

TrendMD:

FU Cuicui, LIANG Lijia, QI Guohua, XU Shuping, XU Weiqing. Biomolecule-assisted Surface-enhanced Raman Scattering(SERS) Technology and SERS Biosensing^†[J]. Chem. J. Chinese Universities, 2015, 36(11): 2134.

Figures/Tables 21

Fig.1 SPR-SERS spectroscopy for detecting bio-identification under silver colloid enhancement[18] Copyright from the Royal Society of Chemistry.

Fig.2 SPR curves of the layer-by-layer assembly of avidin and Atto610-biotin under silver colloid enhancement(A) and SPR curve and the plot of SERS intensities of Atto610-biotin(2.0 mg/mL) captured by avidin on silver film at different incident angles with silver colloid enhancement(B)[18] Curves a—f correspond to the process(A)—(F) in Fig.1. Copyright from the Royal Society of Chemistry.

Fig.3 SPR-SERS spectroscopy for detecting chymotrypsin-catalyzed reaction[19] Copyright from the Royal Society of Chemistry.

Fig.4 SERS spectra of the peptide reacting with different concentrations of chymotrypsin for 90 min[19] Copyright from the Royal Society of Chemistry.

Fig.5 Analysis of the specificity of the SPR-SERS biosensor[19] Copyright from the Royal Society of Chemistry.

Fig.6 Schematic Diagram of the Fabrication of the DNAzyme-Based Plasmonic Nanomechine and Its Mechanism for the Detection of Pb2+ [29] Copyright from the American Chemical Society.

Fig.7 SERS spectra of 4-ATP(1.0 × 10-4 mol/L) with different concentrations of Pb2+ ions[29] Concentration of Pb2+/(mol·L-1): a. 1.0 × 10-6; b. 1.0 × 10-7; c. 1.0× 10-8; d. 1.0 × 10-9; e. 0. Copyright from the American Chemical Society.

Fig.8 Selectivity of the DNAzyme-based SERS biosensor for Pb2+detection [29] Copyright from the American Chemical Society.

Fig.9 Schematic illustration for the SERS detection based on the nanoporous waveguide(PAA) film[34] Copyright from the Elsevier.

Fig.10 Typical reflection spectra(A) of the PAA waveguide layer before(a) and after the adsorptions of human IgG(b), FITC-anti IgG(c) and SERS detection of FITC-anti IgG with different concentrations of IgG through bioidentification by waveguide-assisted SERS method(B)[34] Copyright from the Elsevier.

Fig.11 Schematic diagram of the fabrication of the aptamer-based SERS microfluidic sensor for the detection of PCB77[44] Copyright from the American Chemical Society.

Fig.12 Comparison of SERS spectra of PCB77(1.0×10-7 mol/L) in microfluidic sensor with(a) and without(b) the aptamer[44] Copyright from the American Chemical Society.

Fig.13 Specificity test for the aptamer-based SERS microfluidic sensor with 1.0×10-6 mol/L PCB[44] Insert compares the SERS intensities of PCB77, PCB5, and PCB15 according to the 1288 cm-1 peak[44]. Copyright from the American Chemical Society.

Fig.14 SERS spectra of different concentrations of PCB77(A) and SERS intensity at 1288 cm-1 as a function of concentration of PCB77(B)[44] Copyright from the American Chemical Society.

Fig.15 Glucose-sensing mechanism based on a “turn-off” SERS response

Fig.16 Schematic diagram of our Raman platform with three working modes[64] (A) Dark-field imaging; (B) fluorescence imaging; (C) Raman detection. Copyright from the american chemical society.

Fig.17 Illustration of SGC-7901 cell treated with nuclear targeting nanoprobes and two model drugs[64] Copyright from the American Chemical Society.

Fig.18 Major changes of SERS spectra in the nucleus before and after drug action[64] (A), (C) Dark-field/fluorescence coimages of cancer cells incubated with NLS-PEG-AuNRs and drugs(Hoechst and Dox); (B), (D) mean SERS spectra of cancer cells before and after treatment with drugs, and SERS spectra of Hoechst and Dox; (E) major changes of SERS spectra in the nucleus after drug action. Copyright from the American Chemical Society.

Fig.19 Raman spectra and SHINERS spectrum detection of breast tissue[70] Copyright from the Springer.

Fig.20 Mean spectra of breast tissue(A), optical images of HE tissue sections(B), Raman spectrum detection of breast tissue(C) and optical images of the breast tissue detected by SHINERS spectroscopy(D)[70] The photo shows an area of 0.8 mm×0.6 mm. Copyright from the Springer.

Fig.21 Raman and SERS spectra(A1—A3) of FD, ADH and DCIS containing calcified fibroadenomas, precancerous lesions and in situ duct and optical images(B, C) [71] (B1)—(B3) Breast tissue with calcification in Raman spectra; (C1)—(C3) breast tissue with calcification in SHINERS spectra. The photo shows an area of 0.8 mm×0.6 mm. Copyright from the Elsevier.

References 71

[1]	Kirsch J., Siltanen C., Zhou Q., Chem. Soc. Rev., 2013, 42(22), 8733—8768
[2]	Raman C. V., Krishnan K. S., Nature, 1928, 121(3048), 501—504
[3]	Fleischmann M., Hendra P. J., McQuillan A. J., Chem. Phys. Lett., 1974, 26(2), 163—166
[4]	Jeanmaire D. L., Van Duyne R. P., J. Electroanal. Chem., 1977, 84(1), 1—20
[5]	Wu Z. T., Liu Y. Z., Zhou X. D., Shen A. G., Hu J. M., J. Anal. Sci., 2014, 30(6), 829—839
	(伍子同, 刘翼振, 周晓东, 沈爱国, 胡继明. 分析科学学报, 2014, 30(6), 829—839)
[6]	Zheng J. W., Li X. W., Xu H. Y., Zhou Y. G., Gu R. A., Spectrosc. Spect. Anal., 2003, 23(2), 294—296
	(郑军伟, 李晓伟, 徐浩元, 周耀国, 顾仁敖. 光谱学与光谱分析, 2003, 23(2), 294—296)
[7]	Ge M., Yao J. L., Cui Y., Jiang Y., Gu R. A., Chem. J. Chinese Universities, 2007, 28(8), 1464—1468
	(葛明, 姚建林, 崔颜, 蒋芸, 顾仁敖. 高等学校化学学报, 2007, 28(8), 1464—1468)
[8]	Bao F., Yao J. L., Gu R. A., Langmuir, 2009, 25(18), 10782—10787
[9]	Chen S., Yuan Y. X., Yao J. L., Han S. Y., Gu R. A., Chem. Commun., 2011, 47(14), 4225—4227
[10]	Cui Y., Ren B., Tian Z. Q., J. Southeast Univ.(MedSci. Edi.), 2011, 30(1), 254—262
	(崔颜, 任斌, 田中群. 东南大学学报(医学版), 2011, 30(1), 254—262)
[11]	Huang J., Guo M., Ke H. T., Zong C., Ren B., Liu G., Shen H., Ma Y. F., Wang X. Y., Zhang H. L., Deng Z. W., Chen H. B., Zhang Z. J., Adv. Mater., 2015, 27(34), 5049—5056
[12]	Bantz K. C., Meyer A. F., Wittenberg N. J., Im H., Kurtulus O., Lee S. H., Lindquist N. C., Oh S. H., Haynes C. L., Phys. Chem. Chem. Phys., 2011, 13, 11551—11567
[13]	Futamata M., Keim E., Bruckbauer A., Schumacher D., Otto A., Appl. Surf. Sci., 1996, 100/101, 60—63
[14]	Liu D. L., Zhao Q., Lu D. F., Qi Z. M., Chem. J. Chinese Universities, 2014, 35(10), 2207—2213
	(刘德龙, 赵乔, 逯丹凤, 祁志美. 高等学校化学学报, 2014, 35(10), 2207—2213)
[15]	Yih J. N., Chen S. J., Huang K. T., Su Y. T., Lin G. Y., Proc SPIE., 2004, 5327, 5—9
[16]	Liu Y., Xu S. P., Tang B., Wang Y., Zhou J., Zheng X. L., Zhao B., Xu W. Q., Rev. Sci. Instrum., 2010, 81(3), 036105
[17]	Liu Y., Xu S. P., Li H. B., Jian X. G., Xu W. Q., Chem. Commun., 2011, 47(13), 3784—3786
[18]	Fu C. C., Hu C. X., Liu Y., Xu S. P., Xu W. Q., Anal. Method, 2012, 4(10), 3107—3110
[19]	Fu C. C., Xu W. Q., Chen G., Xu S. P., Analyst, 2013, 138(21), 6282—6286
[20]	Fang Y., Seong N., Dlott D. D., Science, 2008, 321(5887), 388—392
[21]	Xu H., Aizpurua J., Käll M., Apell P., Phys. Rev. E, 2000, 62(3), 4318—4324
[22]	Wang X., Li M., Meng L., Lin K., Feng J., Huang T., Yang Z., Ren B., ACS Nano, 2014, 8(1), 528—536
[23]	Stöckle R. M., Suh Y. D., Deckert V., Zenobi R., Chem. Phys. Lett., 2000, 318(1—3), 131—136
[24]	Halas N. J., Lal S., Chang W. S., Link S., Nordlander P., Chem. Rev., 2011, 111, 3913—3961
[25]	Xuan X. Y., Xu S. P., Liu Y., Li H. B., Xu W. Q., Lombardi John R., J. Phys. Chem. Lett., 2012, 3(19), 2773—2778
[26]	Hermann T., Patel D. J., Science, 2000, 287(5454), 820—825
[27]	Kim N. H., Lee S. J., Moskovits M., Adv. Mater., 2011, 23(36), 4152—4156
[28]	Zheng J., Jiao A., Yang R., Li H., Li J., Shi M., Ma C., Jiang Y., Deng L., Tan W., J. Am. Chem. Soc., 2012, 134(49), 19957—19960
[29]	Fu C. C., Xu W. Q., Wang H. L., Ding H., Liang L. J., Cong M., Xu S. P., Anal. Chem., 2014, 86(23), 11494—11497
[30]	McKee K. J., Meyer M. W., Smith E. A., Anal. Chem., 2012, 84(21), 9049—9055
[31]	Meyer M. W., McKee K. J., Nguyen V. T., Smith E. A., J. Phys. Chem. C, 2012, 116(47), 24987—24992
[32]	Hu D. B., Qi Z. M., J. Phys. Chem. C, 2013, 117(31), 16175—16181
[33]	Gu Y. J., Xu S. P., Li H. B., Wang S. Y., Cong M., Lombardi J. R., Xu W. Q., J. Phys. Chem. Lett., 2013, 4(18), 3153—3157
[34]	Fu C. C., Gu Y. J., Wu Z. Y., Wang Y. Y., Xu S. P., Xu W. Q., Sens. Actuators B, 2014, 201, 173—176
[35]	Manz A., Graber N., Widmer H. M., Sens. Actuators B, 1990, 1(1—6), 244—248
[36]	Thorsen T., Maerkl S. J., Quake S. R., Science, 2002, 298(5593), 580—584
[37]	Whitesides G. M., Nature, 2006, 442(7101), 368—373
[38]	Ríos Á., Zougagh M., Avilaa M., Anal. Chim. Acta, 2012, 740, 1—11
[39]	Chen G., Wang Y. Y., Wang H. L., Cong M., Chen L., Yang Y. A., Geng Y. J., Li H. B., Xu S. P., Xu W. Q., RSC Adv., 2014, 4(97), 54434—54440
[40]	Choi N., Lee K., Lim D. W., Lee E. K., Chang S. I., Oh K. W., Choo J., Lab Chip, 2012, 12(24), 5160—5167
[41]	Lee M., Lee K., Kim K. H., Oh K. W., Choo J., Lab Chip, 2012, 12(19), 3720—3727
[42]	Lu X., Samuelson D. R., Xu Y., Zhang H., Wang S., Rasco B. A., Xu J., Konkel M. E., Anal. Chem., 2013, 85(4), 2320—2327
[43]	Malfatti L., Falcaro P., Marmiroli B., Amenitsch H., Piccinini M., Falqui A., Innocenzi P., Nanoscale, 2011, 3(9), 3760—3766
[44]	Fu C. C., Wang Y., Chen G., Yang L. Y., Xu S. P., Xu W. Q., Anal. Chem., 2015, 87(19), 9555—9558
[45]	Wang X., Xu S., Cong M., Li H., Gu Y., Xu W., Small, 2012, 8(7), 972—976
[46]	Wang Y., Wang Y., Wang H., Cong M., Xu W., Xu S., Phys. Chem. Chem. Phys., 2015, 17(2), 1173—1179
[47]	Shafer-Peltier K. E., Haynes C. L., Glucksberg M. R., Van Duyne R. P., J. Am. Chem. Soc., 2003, 125(2), 588—593
[48]	Haynes C. L, Yonzon C. R, Zhang X., Van Duyne R. P., J. Raman Spectrosc., 2005, 36, 471—484
[49]	Yonzon C. R., Haynes C. L., Zhang X., Walsh J. T., Van Duyne R. P., Anal. Chem., 2004, 76(1), 78—85
[50]	Yonzon C. R., Stuart D. A., Zhang X., McFarland A. D., Haynes C. L., Van Duyne R. P., Talanta, 2005, 67(3), 438—448
[51]	Lyandres O., Shah N. C., Yonzon C. R., Walsh J. T., Glucksberg M. R., Van Duyne R. P., Anal Chem., 2005, 77(19), 6134—6139
[52]	Yang X., Yu Y., Gao Z., ACS Nano, 2014, 8(5), 4902—4907
[53]	Xia Y., Ye J., Tan K., Wang J., Yang G., Anal. Chem., 2013, 85(13), 6241—6247
[54]	Kong K. V., Lam Z., Lau W. K. O., Leong W. K., Olivo M., J. Am. Chem. Soc., 2013, 135(48), 18028—18031
[55]	Yehezkeli O., Tel—Vered R., Raichlin S., Willner I., ACS Nano, 2011, 5(3), 2385—2391
[56]	Qi G. H., Jia K. Q., Fu C. C., Xu S. P., Xu W. Q., J. Opt., 2015, 17, 114020-1—6
[57]	Qian X., Peng X. H., Ansari D. O., YinGoen Q., Chen G. Z., Shin D. M., Yang L., Young A. N., Wang M. D., Nie S., Nat. Biotechnol., 2008, 26(1), 83—90
[58]	Raj L., Ide T., Gurkar A. U., Foley M., Schenone M., Li X., Tolliday N. J., Golub T. R., Carr S. A., Shamji A. F., Stern A. M., Mandinova A., Schreiber S. L., Lee S. W., Nature, 2012, 481(7382), 534—534
[59]	Bair J. S., Palchaudhuri R., Hergenrother P. J., J. Am. Chem. Soc., 2010, 132(15), 5469—5478
[60]	Panikkanvalappil S. R., Mackey M. A., El-Sayed M. A., J. Am. Chem. Soc., 2013, 135(12), 4815—4821
[61]	Panikkanvalappil S. R., Mahmoud M. A., Mackey M. A., El-Sayed M. A., ACS Nano, 2013, 7(9), 7524—7533
[62]	Kang B., Austin L. A., El-Sayed M. A., ACS Nano, 2014, 8(5), 4883—4892
[63]	Li H., Wang H., Huang D., Liang L., Gu Y., Liang C., Xu S., Xu W., Rev. Sci. Instrum., 2014, 85(5), 056109-1—3
[64]	Liang L., Huang D., Wang H., Li H., Xu S., Chang Y., Li H., Yang Y., Liang C., Xu W., Anal. Chem., 2015, 87(4), 2504—2510
[65]	Chen W. Q., Zheng R. S., Zhang S. W., Zhao P., Li G. L., Wu L. Y., He J., Chin J. Cancer Res., 2013, 25(1), 10—21
[66]	Hu C. X., Zheng C., Zhang H. P., Bi L. R., Xu S. P., Fan Z. M., Han B., Xu W. Q., Chem. J. Chinese Universities, 2013, 34(12), 2721—2727
	(胡成旭, 郑超, 张海鹏, 毕丽荣, 徐抒平, 范志民, 韩冰, 徐蔚青. 高等学校化学学报, 2013, 34(12), 2721—2727)
[67]	Zheng C., Zhang H. P., Han B., Liang L. J., Zou Y. B., Xu S. P., Lin H. P., Xu W. Q., Fan Z. M., Chem. J. Chinese Universities, 2015, 36(1), 74—78
	(郑超, 张海鹏, 韩冰, 梁丽佳, 邹亚斌, 徐抒平, 林和平, 徐蔚青, 范志民. 高等学校化学学报, 2015, 36(1), 74—78)
[68]	Anema J. R., Li J. F., Yang Z. L., Ren B., Tian Z. Q., Annu. Rev. Anal. Chem., 2011, 4, 129—150
[69]	Li J. F., Huang Y. F., Ding Y., Yang Z. L., Li S. B., Zhou X. S., Fan F. R., Zhang W., Zhou Z. Y., Wu Y., Ren B., Wang Z. L., Tian Z. Q., Nature, 2010, 464(7287), 392—395
[70]	Zheng C., Liang L., Xu S., Zhang H., Hu C., Bi L., Fan Z., Han B., Xu W., Anal. Bioanal.Chem., 2014, 406(22), 5425—5432
[71]	Liang L. J., Zheng C., Zhang H. P., Xu S. P., Zhang Z., Hu C. X., Bi L. R., Fan Z. M., Han B., Xu W. Q., Spectrochim Acta Part A, 2014, 132, 397—402