Preparation and Characterization of Cationic Chitosan-modified Poly(D,L-|lactide-co-glycolide) Copolymer Nanospheres

Abstract

Abstract: Chitosan(CHS)-modified poly(D,L-lactide-co-glycolide) copolymer(PLGA)(PLGA/CHS) nanospheres with cationic surface were prepared by emulsion-solvent-diffusion(O/W) method. The preparation conditions were optimized by orthogonal factorial design. The diameter of PLGA/CHS nanospheres is in the range of 150 nm to 200 nm, and the ζ potential of PLGA/CHS nanospheres was up to 55 mV when pH was 4. The most important factor which affects the diameter of PLGA/CHS nanospheres is the concentrations of PLGA solutions, whereas the major factors which affect the ζ potential of PLGA/CHS nanospheres are the concentrations of CHS solutions and the molecular weight of CHS(M_{w, CHS}). On the other hand, the pH-value of aqueous phase also affected the ζ potential of PLGA/CHS nanospheres obviously. The optimized conditions for preparing the smallest diameter of PLGA/CHS nanospheres with a higher positive potential were: ρ(CHS)=3 mg/mL, ρ(PLGA)=10 mg/mL, V_o/V_a=1/4 SEM micrograph reveals that the morphology of PLGA/CHS nanospheres was regular. And the results of fluorescence inverted micrograph and XPS analysis indicate that each of the PLGA nanospheres was encapsulated by CHS. The information obtained from this study is useful for preparing CHS-modified particles and the cationic nanospheres hold promise as injectable spheres that are suitable for drug delivery, gene transfection and tissue engineering applications.

Key words: Cationic nanospheres, Poly(D,L-lactide-co-glycolide) copolymer, Chitosan

CLC Number:

O631

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GUAN Xi-Peng, QUAN Da-Ping, LIAO Kai-Rong, MAI Kan-Cheng. Preparation and Characterization of Cationic Chitosan-modified Poly(D,L-|lactide-co-glycolide) Copolymer Nanospheres
[J]. Chem. J. Chinese Universities, doi: .

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Preparation and Characterization of Cationic Chitosan-modified Poly(D,L-|lactide-co-glycolide) Copolymer Nanospheres

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