Preparation of Adsorbents for Endotoxin Removal in Hemoperfusion withAmino Acid as the Ligand and Investigations on Their Properties

Abstract

Abstract: Endotoxermia is caused by endotoxin, lipopolysaccharides(LPS) released from gram-negative bacteria cell wall. Endotoxin is composed of a hydrophilic polysaccharide moiety and a hydrophobic lipid moiety(lipid A) that anchors the molecules in the outer membrane. The sugar residues may be partially phosphorylated and serious endotoxin intoxication can cause sepsis and septic shocks, leading to severe hypertension, cardiovascular collapse, multiple organ failure and death. Removal of endotoxin duly and effectually from patients' blood is very important in clinic. In this paper, with agar gel as the carrier and amino acids as the ligands, adsorbents for endotoxin were prepared and their adsorption capacity for endotoxin were compared. The properties of adsorbents were investigated. The adsorbent with L-lysine as the ligand was chosen to be used for hemoperfusion. The adsorbent can remove plasma endotoxin of endotoxemia rabbit model to close to the normal level and the highest clearance percentage reached 73.6%. The adsorbent has a good blood compatibility.

Key words: Endotoxin, Agar gel, Amino acid, Hemoperfusion

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HOU Guang-Hui, WANG Hui-Yan, LIU Tao, YUAN Zhi, FANG Jie, LI Dong-Hua, QIU Qi, GUO Shi-Duo . Preparation of Adsorbents for Endotoxin Removal in Hemoperfusion withAmino Acid as the Ligand and Investigations on Their Properties[J]. Chem. J. Chinese Universities, 2005, 26(7): 1277.

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Preparation of Adsorbents for Endotoxin Removal in Hemoperfusion withAmino Acid as the Ligand and Investigations on Their Properties

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