Complex and in vitro Release of Methotrexate-PAMAM Dendrimer

Abstract

Abstract: We investigated the interaction between G5.0 PAMAM and methotrexate(MTX) drug molecules by ¹³C NMR, and it has been proven that the interaction is occurring between the PAMAM amine functional groups and the MTX carboxyl groups. UV-Vis study showed each G5.0 PAMAM could conjugate up to 27 MTX molecules. The release of MTX from complex is much slower than that of free MTX at pH=7.4, 10 mmol/L Tris-HCl buffer. The presence of 0.02 mol/L NaCl in buffer increases the release rate of MTX from the complex, which further defines the interaction between PAMAM and MTX.

Key words: Polyamidoamine(PAMAM) dendrimer, Methotrexate(MTX), Release

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YE Ling, JING Xiao-Zhou, YANG Hua, SU Jian-Ting, YUN Liu-Hong . Complex and in vitro Release of Methotrexate-PAMAM Dendrimer[J]. Chem. J. Chinese Universities, 2005, 26(2): 353.

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Complex and in vitro Release of Methotrexate-PAMAM Dendrimer

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