Probing Free Radical Damage to Biological Molecules by Spin-labeling Fluorophore

Abstract

Abstract: A spin-labeling fluorophore(NA-TEMPO^˙), formed by labeling α-naphthalene with a paramagnetic nitroxide(4-hydroxyl-2,2,6,6-tetramethylpiperidinyl-1-oxy), was explored for biologically relevant carbon-centered radicals. The horseradish peroxidase (HPR)/H₂O₂ reaction was used to generate radicals in derivatives of tyrosine and phenylalanine, and the Fenton reaction was used to generate radicals in deoxyguanosine, guanosine, bovine serum albumin(BSA) and DNA. TEMPO ·-NA, a weak fluorescent compound, showed a dramatic increase in fluorescence intensity after scavenging carbon-centered radicals. The spin-labeling fluorophore with dual function groups, the radical recognizing group and fluorescent group, would be significantly useful in monitoring the formation and translocation of carbon-centered radicals in complex biological systems.

Key words: Spin-labeling, Fluorescent probe, Oxidative damage, Carbon-centered radical

CLC Number:

O657

TrendMD:

DANG Ya-Min, GUO Xiang-Qun, ZHAO Yi-Bing . Probing Free Radical Damage to Biological Molecules by Spin-labeling Fluorophore[J]. Chem. J. Chinese Universities, 2004, 25(7): 1245.

References

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