Studies on Adsorption Performance of Affinity Adsorbent for Bacterial Endotoxins

Abstract

Abstract: Bacterial endotoxins(ET) are recognized as the principal mediators in endotoxin shock and multiple organ failure(MOF). Removal of ET in blood by hemoperfusion is one of the effective methods to remove the pathogenic toxin. In the present study,8 amino acids and a poly-L-Lys immobilized on cellulose beads were used in static adsorption experiments to adsorb ET in water solution. Adsorption capacity,adsorption isotherm and affinity mechanism of immobilized amino acid on adsorbents were investigated. The experimental results show that the ligands,Arg and Lys have the highest adsorption capacity which are 182.0 and 160.0 pg/mL gel in 100.0 pg/mL ET solution respectively. Adsorption isotherm of Lys ligand shows that the adsorption amounts of ET increased linearly with increased ET concentrations which shows a good adsorption capacity. The Lys immobilized adsorbent showed to have a high potential for clinical application in endotoxin removal from patients.

Key words: Endotoxin, Hemoperfusion, Cellulose bead, Amino acid

CLC Number:

Q786

TrendMD:

FANG Hui, WEI Jiao, YU Yao-Ting. Studies on Adsorption Performance of Affinity Adsorbent for Bacterial Endotoxins[J]. Chem. J. Chinese Universities, 2004, 25(6): 1056.

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Studies on Adsorption Performance of Affinity Adsorbent for Bacterial Endotoxins

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