A Biosensor Based on Immobilizing Horseradish Peroxidase on SiO2 Nanoparticles

Abstract

Abstract: Horseradish peroxidase(HRP) was immobilized on SiO₂(about 60 nm) nanoparticles. In this experiment, HRP can remain a higher bioactivity with the Michealis-Menten constant of 7.8 μmol/L . By means of catechol mediator in the solution, HRP/SiO₂ modified electrode displayed an excellent electrocatalytical response linearly to the reduction of H₂O₂ in the concentration range of 1.7×10^-7 —1.9×10^-5mol/L. The detection limit was 8.3×10^-8mol/L. The time for steady-state current reaching 95% is less than 10 s.

Key words: SiO₂ nanaparticles, HRP, Biosensor

CLC Number:

O657

TrendMD:

ZHANG Juan, XU Jing-Juan, CHEN Hong-Yuan . A Biosensor Based on Immobilizing Horseradish Peroxidase on SiO₂ Nanoparticles[J]. Chem. J. Chinese Universities, 2004, 25(4): 614.

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A Biosensor Based on Immobilizing Horseradish Peroxidase on SiO₂ Nanoparticles

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