Glucose Biosensor Based on Rebuilding the Surface of the Spiral-type Pt-Ir Electrode†

doi:10.7503/cjcu20160777

Abstract

Abstract:

The surface of the spiral-type platinum-iridium electrode was rebuilt and optimized by chemical etching and electrochemical deposition of platinum nanoparticles(PtNPs). The effects of different etching time and electrodeposition time on the morphology and the catalytic activity of the electrode toward hydrogen peroxide were studied. After coating glucose oxidase(GOD) and epoxy-polyurethanes(Epoxy-PU) to surface-rebuilt electrode, the PtNPs/GOD/Epoxy-PU enzymatic biosensor was successfully fabricated. The chronoamperometric test indicates that the detection range of PtNPs/GOD/Epoxy-PU biosensor is expanded to 2—45 mmol/L, which is much wider than that of bare electrode based biosensor. PtNPs/GOD/Epoxy-PU biosensor has high response current and sensitivity, good stability and selectivity as well.

Key words: Glucose biosensor, Spiral-type platinum-iridium electrode, Chemical etching, Electrochemical deposition, Detection range(Ed.: S, Z, M)

CLC Number:

O657.1

TrendMD:

PAN Shuai, LI Zhanhong, CHEN Yang, ZHAO Xueling, CHEN Cheng, ZHU Zhigang. Glucose Biosensor Based on Rebuilding the Surface of the Spiral-type Pt-Ir Electrode^†[J]. Chem. J. Chinese Universities, 2017, 38(7): 1163.

Figures/Tables 8

Fig.1 Schematic diagram of the spiral-type Pt-Ir electrode

Fig.2 Chronoamperometry responses of the spiral-type Pt-Ir electrode with different etching time to hydrogen peroxide(at 0.5 V vs. Ag/AgCl reference)(A), hydrogen peroxide sensitivity for the spiral-type Pt-Ir electrode with different etching time(B) and cyclic voltammograms of the spiral-type Pt-Ir electrode in 0.4 mmol/L K3(FeCN6)/0.1 mol/L KCl aqueous solution at 50 mV/s scanning rate with different etching time(C)Etching time/min: a. 0; b. 15; c. 30; d. 60.

Fig.3 SEM images of the spiral-type Pt-Ir electrodes Etching time/min: (A) 0; (B) 15; (C) 30; (D) 60.

Fig.4 Chronoamperometry responses of etched spiral-type Pt-Ir electrode with different electrodeposition time to hydrogen peroxide(at 0.5 V vs. Ag/AgCl)(A) and cyclic voltammograms of etched electrode in 0.1 mol/L H2SO4 aqueous solution at 50 mV/s scanning rate with different electrodeposition time(B) and hydrogen peroxide sensitivity for etched electrode with different electrodeposition time(C)

Table 1 Mass specific activity and active surface area of the spiral-type Pt-Ir electrode with different electrodeposition time

Etching time/min	Electro-deposition time/min	Deposition amount of platinum nanoparticles/mg	Active surface area/cm²	Activity surface area/ Deposition amount of Pt/(cm²·mg^-1)	Increased time of active surface area^*
0	0	0	0.039	0	0
30	5	0.28	0.489	1.75	12.54
30	10	0.41	0.786	1.92	19.15
30	15	0.79	1.026	1.30	25.30
30	20	1.11	1.070	0.96	26.44
30	25	1.50	1.435	0.95	36.80

Fig.5 SEM images of the etched spiral-type Pt-Ir electrode with various electrochemical deposition time Deposition time/min: (A) 0; (B) 5; (C) 10; (D) 15; (E) 20; (F) 25.

Fig.6 Amperometric response of etched PtNPs/GOD/Epoxy-PU electrode(a) and GOD/Epoxy-PU electrode(b) in different concentrations of glucose solution(A) and linear response curves of etched PtNPs/GOD/Epoxy-PU electrode(a) and GOD/Epoxy-PU electrode(b)(B)

Fig.7 Amperometric response(A) and sensitivity changes(B) of etched PtNPs/GOD/Epoxy-PU electrode within four weeks and sensor response to sequential additions of 5 mmol/L glucose, 0.5 mmol/L AA, 0.5 mmol/L DA, 0.5 mmol/L UA, 0.5 mmol/L F and 5 mmol/L glucose(C)

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