Studies on an Electrochemical DNA Biosensor Based on Gold Nanoparticle-labeled DNA Probe

Abstract

Abstract: An electrochemical detection method for the analyzing sequence-specific DNAusing gold nanoparticle labeled oligonucleotide DNAprobes has been developed.Target DNAwere covalently immobilized on a glass carbon electrode and incubated with gold nanoparticle-labeled DNAprobes, containing a complementary sequence.Binding events were monitored by electrochemical signal of gold nanoparticle hybridized to the electrode surface.The signal difference permits to distinguish the match of two perfectly complementary DNAstrands from the near-perfect match where just one base pair was wrong.The sensitivity of this electrochemical DNAbiosensor has been detected and a detection limit of 1.0 nmol/Lof oligonucleotides was achieved.

Key words: Gold nanoparticle, Electrochemical DNA biosensor, Hybridization, Mismatch detection

CLC Number:

O657

TrendMD:

CAI Hong, WANG Yan-Qin, HE Pin-Gang, FANG Yu-Zhi . Studies on an Electrochemical DNA Biosensor Based on Gold Nanoparticle-labeled DNA Probe[J]. Chem. J. Chinese Universities, 2003, 24(8): 1390.

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Studies on an Electrochemical DNA Biosensor Based on Gold Nanoparticle-labeled DNA Probe

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