A Phenomenon of Negative Peak Appeared in Kinetic Ultraviolet Absorb Curve of Initial Biomineralization

Abstract

Abstract: The ultraviolet photometry is an effective method to investigate the kinetic process of biomineralization because it can be used to monitor the reaction in real time without disturbance. This paper reports an interesting phenomenon in experiment on collagen mineralization. An abnormal negative peak was found in kinetic ultraviolet absorb curve of collagen/calcium phosphate biomineralization system in Tris-HCl buffer(pH=7.4). Influence of several experiment conditions on the negative peak was studied. SEM observation of the sample before and after the negative peak revealed that the crystals morphology and the aggregation of the mineralized collagen fibers are different. It indicated that the negative peak in the kinetic curve is an optical phenomenon related to the compositional and structural change of the mineral, which are the important elements in biomineralization mechanism study.

Key words: Biomineralization, Ultraviolet spectrophotometer, Collagen, Calcium phosphate

CLC Number:

O657.31

TrendMD:

HUANG Zhao-Long, ZHANG Wei, CUI Fu-Zhai, CAI Qiang . A Phenomenon of Negative Peak Appeared in Kinetic Ultraviolet Absorb Curve of Initial Biomineralization[J]. Chem. J. Chinese Universities, 2003, 24(6): 1013.

[1]	LIU Guomin, LU Tiancheng, JI Xuan, JIA Wenyuan, LI Yalong, ZHAO Yian, LUO Yungang. Preparation and Osteogenic Induction Activity of CBD-BMP2-MP/PLGA 3D Printed Composite Scaffolds^† [J]. Chem. J. Chinese Universities, 2019, 40(7): 1552.
[2]	ZHOU Ying, WANG Xianliu, YI Bingcheng, YU Zhepao, YANG Shangying, SHEN Yanbing, ZHANG Yanzhong. Engineering Shape Memory Enabled Composite Nanofibers for Bone Tissue Engineering^† [J]. Chem. J. Chinese Universities, 2018, 39(7): 1554.
[3]	HUANG Run, PAN Chengling*, ZHANG Lan. Effect of Surface Mechanical Attrition Treatment on Bioactivity of Biomedical Titanium Alloy^† [J]. Chem. J. Chinese Universities, 2017, 38(4): 522.
[4]	CUI Guolian, DAN Nianhua, DAN Weihua. Preparation and Characterization of Novel Dopamine-based Bioadhesive Hydrogels^† [J]. Chem. J. Chinese Universities, 2017, 38(2): 318.
[5]	BU Honghong, JIAO Yanni, LAN Wenjun, ZHUANG Xiaoli, DAI Xiaonan, LI Tianduo. Rheological Properties of GuHCl/Type Ⅰ Collagen Dispersions^† [J]. Chem. J. Chinese Universities, 2016, 37(6): 1189.
[6]	GAN Qiongzhi, SUN Xinyuan, YAO Xiuqiong, OUYANG Jianming. Molecular Mechanism of Adhesion of Monohydrate and Dihydrate Calcium Oxalate Crystals on Injured Kidney Epithelial Cells^† [J]. Chem. J. Chinese Universities, 2016, 37(6): 1050.
[7]	SUN Hui, YU Qingsong, YANG Biao, XU Guozhi. Surface Hydrophilic Modification of Poly(ether ether ketone) and Immobilization of Collagen^† [J]. Chem. J. Chinese Universities, 2016, 37(6): 1154.
[8]	CHEN Lamei, CAO Jie, YE Lin, ZHANG Aiying, FENG Zengguo. Preparation of Multi-functional Wound Dressing with Core-shell Nanofibers via Co-axial Electrospinning [J]. Chem. J. Chinese Universities, 2016, 37(3): 600.
[9]	WU Ranran, TIAN Xiaochun, WU Shenjian, LIU Yuangang, JIANG Yanxia, ZHAO Feng. Research on the Electrochemical Activity of Magnetospirillum Magneticum AMB-1^† [J]. Chem. J. Chinese Universities, 2015, 36(9): 1730.
[10]	XIAO Zhenkun, YU Haoran, WU Tao, WANG Ruizhen, PENG Zhang, LU Xiaoying, WENG Jie. Effects of Adding Reduced-graphene Oxide/Polypyrrole Composites on the Structure and Properties of Calcium Phosphate Cement^† [J]. Chem. J. Chinese Universities, 2015, 36(12): 2598.
[11]	ZHANG Xia, ZHOU Hao, YANG Yuhong, HUANG Yufang, CHEN Xin. Oxidized Cellulose Enhanced Collagen Hydrogels^† [J]. Chem. J. Chinese Universities, 2015, 36(10): 2040.
[12]	WANG Huan, CHEN Ning, WANG Qi. Interaction Between β-Tricalcium Phosphate and Poly(vinyl alcohol) and Its Effect on the Thermal and Mechanical Properties of Poly(vinyl alcohol)^† [J]. Chem. J. Chinese Universities, 2014, 35(8): 1810.
[13]	ZENG Qinghui, LI Na, TIAN Ye, WU Di, ZHAO Yaowu, LI Lihua, ZHOU Changren. Effects of Collagen Films with Liquid Crystal-liked Ordered Structure on Adhesion, Proliferation and Differentiation of Human Umbilical Cord Mesenchymal Stem Cells^† [J]. Chem. J. Chinese Universities, 2014, 35(8): 1658.
[14]	DING Shan, TANG Min-Jian, ZHOU Chang-Ren, MIN Xiang, TIAN Jin-Huan, LI Li-Hua. Synthesis and Properties of Cholesterol Graft Chitosan [J]. Chem. J. Chinese Universities, 2012, 33(05): 1110.
[15]	LI Bo, ZHANG Hui-Jie, LIAO Xiao-Ling, ZHU Xiang-Dong, FAN Hong-Song, ZHANG Xing-Dong. Effects of Microstructure of Calcium Phosphate Ceramics Particles on Protein Adsorption [J]. Chem. J. Chinese Universities, 2012, 33(03): 447.

A Phenomenon of Negative Peak Appeared in Kinetic Ultraviolet Absorb Curve of Initial Biomineralization

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments