Preparation of Multi-functional Wound Dressing with Core-shell Nanofibers via Co-axial Electrospinning

doi:10.7503/cjcu20150671

Abstract

Abstract:

The uncrosslinked wound dressing with core-shell nanofibers was firstly fabricated via co-axial electrospinning. The nanofiber was composed of the polycaprolactone(PCL) core loaded with anti-bacterium drug mupirocin and collagen shell with anesthesia lidocains. The surface morphology of uncrosslinked dressing and its core-shell structured fibers were characterized by scanning electronic microscopy and transmission electronic microscopy, respectively. The crosslinked wound dressing can be further prepared by the crosslinking of collagen shell, and the successful crosslinking can be proved by the enhanced tensile strength and swelling ratio. In vitro drug release tests implied two dressings both possessed the burst release stage and sustainable release stage for two loaded drugs, which confirmed the sustainable acesodyne and anti-bacteria effects for both dressings. The bicinchonininc acid(BCA) test results showed that the collagen in uncrosslinked dressing could also be sustainably released, while in vitro cell attachment test depicted that uncrosslinked dressing could promote the cell attachment and growth better than crosslinked dressing. Furthermore, the in vitro anti-bacteria test showed that both dressings loaded with mupirocin had significantly better anti-bacteria ability than those without mupirocin indicating their excellent anti-bacteria ability. Two as-prepared multifunctional wound dressings possessed acesodyne and anti-bacteria effects and were able to promote the healing process so that they can reduce the pains of patients, reduce the number of dressing change, and enhance treatment effect.

Key words: Co-axial electrospinning, Multifunctional wound dressing, Collagen, In vitro anti-bacteria, Cell attachment

CLC Number:

O631.2

TrendMD:

CHEN Lamei, CAO Jie, YE Lin, ZHANG Aiying, FENG Zengguo. Preparation of Multi-functional Wound Dressing with Core-shell Nanofibers via Co-axial Electrospinning[J]. Chem. J. Chinese Universities, 2016, 37(3): 600.

References

[1] Liu X., Lin T., Gao Y., Xu Z. G., Huang C., Yao G., Jiang L. L., Tang Y. W., Wang X. G., Journal of Biomedical Materials Reseach B:Applied Biomaterials, 2012, 100, 1556-1565
[2] Tan M., Li L. S., Chemistry Bulletin, 2000,(11), 7-12(谈敏, 李临生. 化学通报, 2000,(11), 7-12)
[3] Lalani R., Liu L. Y., Biomacromolecules, 2012, 13, 1853-1863
[4] Morgado P. I., Aguiar-Ricardo A., Correia I. J., Journal of Membrane Science, 2015, 490, 139-151
[5] Abrigo M., McArthur S. L., Kingshott P., Macromolecular Bioscience, 2014, 14, 772-792
[6] Agarwal S., Wendorff J. H., Greiner A., Polymer, 2008, 49, 5603-5621
[7] Zahedi P., Rezaeiana I., Ranaei-Siadatb S. O., Jafaria S. H., Supaphol P., Polym. Adv. Technol., 2010, 21, 77-95
[8] Liu Y., Cao P., He G., Lin Z. S., Qiu T., Li C., Cai X. D., Ao N. J., Polymer Bulletin, 2014,(6), 40-45(刘源, 曹苹, 何桂, 林钟石, 邱涛, 李淳, 蔡信东, 敖宁建. 高分子通报, 2014,(6), 40-45)
[9] Fan Y. N., Cai Z. J., Zhao K. Y., Polymer Bulletin, 2013,(11), 70-75(樊亚男, 蔡志江, 赵孔银. 高分子通报, 2013,(11), 70-75)
[10] Nina L., Afeesh R. U., Mahesh K. J., Arjun P. T., Seong Tshool H., Chan-Hee P., Cheol S. K., Colloids and Surfaces A:Physicochem. Eng. Aspects, 2015, 469, 194-201
[11] Tan L., Hu J. L., Zhao H. F., Materials Letters, 2015, 156, 46-49
[12] Zhou Y. S., Yang D. Z., Chen X. M., Xu Q., Lu F. M., Nie J., Biomacromolecules, 2008, 9, 349-354
[13] Tan L., Hu J. L., Huang H. H., Han J. P., Hu H. W., International Journal of Biological Macromolecules, 2015,(79), 469-476
[14] Tang C., Guan Y.X., Yao S. J., Zhu Z.Q., Acta Polymerica Sinica, 2014,(6), 774-781(唐川, 关怡新, 姚善泾, 朱自强. 高分子学报, 2014,(6), 774-781)
[15] Payam Z., Zeinab K., Iraj R., Seyed-Hassan J., Parvin M., Amir H. A., Mohammad A., Journal of Applied Polymer Science, 2012, 124, 4174-4183
[16] Chen Y., Dou G. F., Luo Y. J., Tan H. M., Polymer Bulletin, 2005,(1), 94-100(陈煜, 窦桂芳, 罗运军, 谭惠民. 高分子通报, 2005,(1), 94-100)
[17] Uyanga D., Mark K. R., Afeesh R. U., Arjun P. T., Batgerel T., Chan H. P., Cheol S. K., International Journal of Biological Macromolecules, 2015,(80), 1-7
[18] Tchemtchoua V. T., Atanasova G., Aqil A., Filee P., Garbacki N., Deroanne C., Noel A., Jerome C., Nusgens B., Biomacro-molecules, 2011, 12, 3194-3204
[19] Gao Y., Truong Y. B., Zhu Y. G., Kyratzis I. L., Journal of Applied Polymer Science, 2014, DOI:10.1002/app.40797
[20] He T., Wang J. N., Huang P. L., Zeng B. Z., Li H. H., Gao Q. Y., Zhang S. Y., Luo Z., Deng D. Y. B., Zhang H. W., Zhou W. Y., Colloids and Surfaces B:Biointerfaces, 2015, 130, 278-286
[21] Sun B., Study on Electrospun Core/shell Structured Ultrafine Fibers for Wound Dressings, Tianjin University, Tianjin, 2007(孙斌. 芯/壳结构超细纤维及其用作伤口敷料的研究, 天津:天津大学, 2007)
[22] Wu L. L., Study on Electrospun PLGA/chitosan Composite Membranes for Artificial Skin, Tianjin University, Tianjin, 2007(邬丽丽. PLGA壳聚糖电纺复合膜用于人工皮肤的研究, 天津:天津大学, 2007)
[23] Chen L., Study and Fabricate on a Novel Human-like Collagen(HLC)/chitosan Complex Nano Fibrous Scaffold by Electrospinning, Northwest University, Xi'an, 2010(陈岚. 新型类人胶原蛋白-壳聚糖静电纺丝纳米纤维结构组织工程支架的构建与研究, 西安:西北大学, 2010)
[24] Thakur R. A., Florek C. A., Kohn J., International Journal of Pharmaceutics, 2008, 87-93
[25] Xu X. L., Study on the Preparation and Environmentalism Pact of New Wound Dressing-gelatin-based Antibacterial Nanofiber Hydrogel, Donghua University, Shanghai, 2009(徐雄立. 新型医用敷料明胶基抗菌纳米纤维水凝胶的制备及其环境影响研究, 上海:东华大学, 2009)
[26] Fang Y., Yang Z., China Pharmacy, 2013, 24(33), 3143-3144(范莹, 杨昭. 中国药房, 2013, 24(33), 3143-3144)
[27] Dong Y., China Pharmaceuticals, 2010, 19(2), 36-37(董煜. 中国药业, 2010, 19(2), 36-37)