Fluorescence Enhancing Method for the Determination of Human Serum Albumin with Functionalized CdS Nanoparticles as Fluorescence Probes

Abstract

Abstract: CdS nanoparticles were prepared and modified with mercaptoacetic acid. The functionalized nanoparticles were water soluble and biocompatible and used as fluorescence probe in the determination of Human serum albumin(HSA) and γ globulin(γ G). Under the optimal conditions, linear relationships were found between the enhanced intensity of fluorescence and the concentration of protein in the range of 0.2 and 3.5 μg/mL for HSA and 0.2 and 11.0 μg/mL for γ G. The limits of detection were 0.09 μg/mL for HSA and 0.18 μg/mL for γ G.

Key words: CdS Nanoparticle, Fluorescence probe, Human serum albumin

CLC Number:

O614

TrendMD:

WANG Le Yu, ZHOU Yun You, ZHU Chang Qing, ZHANG Ming Cui, TAO Hai Sheng, WANG Lun. Fluorescence Enhancing Method for the Determination of Human Serum Albumin with Functionalized CdS Nanoparticles as Fluorescence Probes[J]. Chem. J. Chinese Universities, 2003, 24(4): 612.

References

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