Studies on Labeling of Papain with Quantum DotsSynthesized in Aqueous Solution

Chem. J. Chinese Universities ›› 2003, Vol. 24 ›› Issue (4): 609.

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Studies on Labeling of Papain with Quantum DotsSynthesized in Aqueous Solution

LIN Zhang Bi¹, ZHANG Hao², CHEN Qi Dan¹, WAN Yi¹, YANG Bai², SU Xing Guang^1,3, ZHANG Jia Hua³, JIN Qin Han¹

1. Institute of Analytical Science, College of Chemistry;
2. Key Lab for Supramolecular Structure and Materials, Ministry of Education, Jilin University, Changchun 130023, China;
3. Laboratory of Excited State Processes, Chinese Academy of Sciences, Changchun 130021, China

Received:2002-11-25 Online:2003-04-24 Published:2003-04-24

Abstract

Abstract: The feasibility of the mercaptopropyl acid modified CdTe nanoparticles synthesized in aqueous solution to the labeling of biomoleculespapain via electrostatic interaction was studied in this paper. The absorption spectrum of CdTe papain solution became more flat than that of CdTe nanoparticles in the wavelength range from 400 to 600 nm. While the emission peak of CdTe papain solution underwent a red shift, the intrinsic emission band width was unchanged. The TEM images of monodisperse mercaptopropyl acid stabilized CdTe nanoparticles and CdTe papain solution were compared and the effect of pH of buffer solution was studied. It was proved that the red shift of the emission peak was the result of the electrostatic interaction between CdTe nanoparticles and papain.

Key words: Quantum dots, Papain, Electrostatic interaction

CLC Number:

O657.31

TrendMD:

LIN Zhang Bi, ZHANG Hao, CHEN Qi Dan, WAN Yi, YANG Bai, SU Xing Guang, ZHANG Jia Hua, JIN Qin Han . Studies on Labeling of Papain with Quantum DotsSynthesized in Aqueous Solution[J]. Chem. J. Chinese Universities, 2003, 24(4): 609.

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Studies on Labeling of Papain with Quantum DotsSynthesized in Aqueous Solution

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