Live-cell Synthesis of ZnSe Quantum Dots in Staphylococcus aureus†

doi:10.7503/cjcu20180127

Abstract

Abstract:

Staphylococcus aureus cells were skillfully utilized to produce ZnSe quantum dots and transformed into fluorescing cells through temporally-spatially coupling strategy. The products were characterized by means of transmission electron microscopy(TEM), atomic force microscope(AFM), dynamic light scattering(DLS), inductively coupled plasma emission spectrometry(ICP-AES), UV-Vis absorption spectroscopy and photoluminescence(PL) spectroscopy. ZnSe QDs biosythesized by such a method possess uniform particle size of (2.96±0.76) nm and good dispersibility with fluorescence emission at 537 nm.

Key words: ZnSe, Quantum dots, Staphylococcus aureus, Live-cell, Biosynthesis

CLC Number:

O657.3

TrendMD:

ZHANG Yanan, YANG Lingling, TU Jiawei, CUI Ran, PANG Daiwen. Live-cell Synthesis of ZnSe Quantum Dots in Staphylococcus aureus^†[J]. Chem. J. Chinese Universities, 2018, 39(6): 1158.

Figures/Tables 6

Fig.1 Images of S. aureus cells cultured with Na2SeO3 and ZnSO4 in bright field(A) and fluorescent field(B), corresponding images for the control group(C, D) and the average hydrodynamic sizes of cells in experiment group(E) and control group(F)

Fig.2 TEM image(A), HRTEM images(B, C), size distribution(D) and hydrodynamic size(E) of isolated nanoparticles from S.aureus

Fig.3 Top view AFM image(A) and height profile(B) of isolated nanoparticles from S.aureus

Fig.4 EDX spectrum of isolated nanoparticles from S. aureus

Fig.5 UV-Vis absorption spectra(A) and photoluminescence emission spectra(B) of isolated ZnSe QDs synthesized in S. aureusa. ZnSe; b. control.

Fig.6 Photostability of isolated ZnSe QDs with continuous illumination from a mercurylamp(A), dispersed in different systems(B) and in the presence of NaCl solution at different concentrations(C)

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