Hydrothermal Synthesis of Ultrasmall Fluorescent Tungsten Disulfide Quantum Dots and Their Application in Cell imaging†

doi:10.7503/cjcu20180635

Abstract

Abstract:

Ultrasmall tungsten disulfide quantum dots(WS₂ QDs) were prepared by one-step hydrothermal method using sodium tungstate and cysteine as starting materials. The WS₂ QDs were characterized by means of transmission electron microscopy(TEM), fluorescence spectroscopy, infrared spectroscopy and X-ray diffraction spectroscopy(XRD), and their stability and cell toxicity were investigated. The results show that the as-prepared WS₂ QDs have good water-solubility, high stability, and low cell toxicity. Then, the WS₂ QDs were used for MCF-7 human breast cancer cells imaging, and the results of fluorescence co-localization imaging of Lyso-Tracker Red probes indicate that the WS₂ QDs enter the cells via lysosomal pathway.

Key words: Tungsten disulfide quantum dots, Cell imaging, Fluorescence

CLC Number:

O657
O611.4

TrendMD:

MU Yaxin,A Li,ZHUANG Qianfen,WANG Yong,NI Yongnian. Hydrothermal Synthesis of Ultrasmall Fluorescent Tungsten Disulfide Quantum Dots and Their Application in Cell imaging^†[J]. Chem. J. Chinese Universities, 2019, 40(4): 693.

Figures/Tables 7

Scheme 1 Hydrothermal synthesis of WS2 QDs and their application in cell imaging

Fig.1 TEM image(A)and size distribution(B)of WS2 QDsInset of (A) is the HRTEM image of WS2 QDs.

Fig.2 XPS spectrum(A), XRD pattern(B) and FTIR spectrum(C) of WS2 QDs

Fig.3 Excitation and emission spectra of WS2 QDs(A)and emission spectra of WS2 QDs with different excitation spectrum(B)Insets of (A) are the optical images of WS2 QDs under daylight(a) and 365 nm UV light(b), respectively.

Fig.4 Effects of pH and NaCl concentration on fluorescence intensity of WS2 QDs(A) and fluorescence intensity of WS2 QDs under continous irradiation for 60 min(B)

Fig.5 Cell viability of MCF-7 incubated with different concentrations of WS2 QDs(0—1000 μg/mL)

Fig.6 Cofocal images of MCF-7 cell incubated with 400 μg/mL of WS2 QDs for different time(0, 1, 3 and 6 h) and co-localization images with the Lyso-tracker Red(A) WS2QDS+0 h; (B) Lyso tracker+0 h; (C) Merge+0 h; (D) WS2QDS+1 h; (E) Lyso tracker+1 h; (F) Merge+1 h; (G) WS2QDS+3 h; (H) Lyso tracker+3 h; (I) Merge+3 h; (J) WS2QDS+6 h; (K) Lyso tracker+6 h; (L) Merge+6 h.

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