Abstract: A RP HPLCmethod for the separation and determination of beryllium(Ⅱ), chromium(Ⅲ), copper(Ⅱ), iron(Ⅱ) and nickel(Ⅱ) with chromotrope 2Cand TANchelates on a YWG ODScolumn by using methanol ethanol water(volume ratio is 35:5:60) containing5×10^-5 mol/Lchromotrope 2C, 5×10^-5 mol/L TANand acetate buffer solution(pH=50) as mobile phase and with spectrophotometric detection at 580 nm was developed. The method has a high sensitivity. The detection limits were 5 μg/mLfor beryllium(Ⅱ), 10 μg/mLfor chromium(Ⅲ), 10 μg/mLfor copper(Ⅱ), 20 μg/mLfor iron(Ⅱ) and 04 μg/mLfor nickel(Ⅱ). Under the optimum conditions most other metal ions do not interfere. The method can be applied to the simultaneous determination of trace amounts of beryllium, chromium, copper, iron and nickel in rice and flour samples.

Key words: Seperation and determination of metal ions, Chromotrope2-C, TAN