Quantitative Determination of Resveratrols in Wine by GC/MS and Elementary Study on Their Physiological Activity

Abstract

Abstract: C₁₈ SPEmethod was applied to the extraction of the resveratrols from Chinese wine. GC/MShas been used to analyze two isomers of resveratrol, cis- and trans-resveratrol in wine simultaneously. Identification was based on retention time and mass ion spectrum. These two compounds were quantitative determined. The precision of the method for the determination of cis and trans resveratrol was 4.48% and 5.23% of RSD, respectively. The recovery of cis and trans resveratrol was 94.4% and 97.6%, respectively. Twelve kinds of actual wine samples were determined. The elementary study was also performed for physiological activity of resveratrols. The effects of resveratrols on growth and reproductive activity of VSMC, LOVO, 3T3 cells of mouse were preliminary researched and the morphological change of these cells had been observed.

Key words: GC/MS, Wine, Resveratrol, Solid phase extraction, MTT, Cellular morphology, Tumor cell

CLC Number:

O657.O626.11

TrendMD:

MA Ting, LI Gong-Ke, LI Xiao-Dong, TIAN Xue-Mei, ZHANG Zhan-Xia, BAO Lun-Jun, ZHOU Hui-Tang. Quantitative Determination of Resveratrols in Wine by GC/MS and Elementary Study on Their Physiological Activity[J]. Chem. J. Chinese Universities, 2000, 21(7): 1023.

[1]	WANG Yuanyue, AN Suosuo, ZHENG Xuming, ZHAO Yanying. Spectroscopic and Theoretical Studies on 5-Mercapto-1，3，4-thiadiazole-2-thione Microsolvation Clusters [J]. Chem. J. Chinese Universities, 2022, 43(10): 20220354.
[2]	ZHENG Haijiao, JIANG Liyan, JIA Qiong. Arginine Functionalized Magnetic Nanoparticles and Its Application in Phosphopeptides Enrichment [J]. Chem. J. Chinese Universities, 2021, 42(3): 717.
[3]	WANG Xiaoru,ZHANG Na,XING Jun. Preparation and Application of Melamine Imprinted Material Using Itaconic Acid as Multidentate Functional Monomer [J]. Chem. J. Chinese Universities, 2020, 41(7): 1521.
[4]	WANG Tianqi,YU Qiongwei,FENG Yuqi. Analysis of Imidazole Propionic Acid in Serum of Patients with Type 2 Diabetes Based on NiO@SiO₂ Solid-phase Extraction Coupled with Liquid Chromatography-Mass Spectrometry ^† [J]. Chem. J. Chinese Universities, 2020, 41(2): 262.
[5]	MENG Zihui,WANG Yifei,XIE Tengsheng,CHEN Wei,QIU Lili,XUE Min. Molecularly Imprinted Hollow Spheres for the Solid Phase Extraction of Protein^† [J]. Chem. J. Chinese Universities, 2019, 40(1): 62.
[6]	YANG Mingming,NAN Lijing,JIN Wanjun,WANG Chengjian,HUANG Linjuan,ZHANG Ying,WANG Zhongfu. Quali-quantitative Profiling of Mucin-type O-Glycans in Normal and Tumor Cell Lines Based on Oligosaccharide Metabolic Engineering Combined with Mass Spectrometry^† [J]. Chem. J. Chinese Universities, 2018, 39(9): 1904.
[7]	WANG Xueli,WANG Zhenxin. Preparation of a Targeted Tumor Nanocomposites for Combined Photodynamic-photothermal Therapy Based on Partially Reduced Graphene Oxide^† [J]. Chem. J. Chinese Universities, 2018, 39(10): 2185.
[8]	ZHANG Jinliang, MA Xin, XU Mengxi, ZONG Cheng, REN Bin. Study on Apoptosis Process of CaSki via Fast Line-scanning Raman Imaging^† [J]. Chem. J. Chinese Universities, 2016, 37(7): 1257.
[9]	YANG Zechuan, LI Fan, HUANG Qingrong, ZHANG Guo, SHI Tongfei. Synthesis and Properties of the Amino Acid Functionalized Curcumin/His-Pectin Colloidal Particles^† [J]. Chem. J. Chinese Universities, 2016, 37(2): 381.
[10]	GUAN Xiao, YIN Ting, HAN Fei. Light Stability, Controlled-release and Antioxidation of Resveratrol-hordein Composite Nanoparticles^† [J]. Chem. J. Chinese Universities, 2015, 36(9): 1707.
[11]	RAO Wei, YIN Yuli, LONG Fang, ZHANG Zhaohui. Preparation and Application of Magnetic Bisphenol A Imprinted Polymers with Binary Functional Monomers^† [J]. Chem. J. Chinese Universities, 2015, 36(3): 449.
[12]	RAO Wei, CAI Rong, CHEN Xing, LIU Yu-Nan, CHEN Hong-Jun, ZHANG Zhao-Hui, NIE Li-Hua. Preparation and Adsorption Properties of Novel Magnetic Tetrabromobisphenol A Molecularly Imprinted Composite Material Based on Graphene Oxide Surface [J]. Chem. J. Chinese Universities, 2013, 34(6): 1353.
[13]	HUO Dan-Qun, FANG Ke-Jing, HOU Chang-Jun, YANG Mei, FA Huan-Bao, HUANG Cheng-Hong, LUO Xiao-Gang. Rapid Fabrication of Hydrogel Microfluidic Chip and Its Application for Cell Metabolites Detection [J]. Chem. J. Chinese Universities, 2013, 34(6): 1327.
[14]	CAO Hui, CHEN Xiao-Zhen, ZHU Yan, LI Zu-Guang. Simultaneous Determination of Multi-veterinary Drug Residues in Honey by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry and Multiwalled Carbon Nanotubes Cleaning [J]. Chem. J. Chinese Universities, 2013, 34(12): 2710.
[15]	ZHANG Jian-Cheng, DING Jian-Xun, XIAO Chun-Sheng, HE Chao-Liang, ZHUANG Xiu-Li, YANG Ya-Nan, CHEN Xue-Si. Synthesis and Characterization of Tumor-acidity-sensitive Poly(L-lysine)-doxorubicin Conjugates [J]. Chem. J. Chinese Universities, 2012, 33(12): 2809.

Quantitative Determination of Resveratrols in Wine by GC/MS and Elementary Study on Their Physiological Activity

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments