Light Stability, Controlled-release and Antioxidation of Resveratrol-hordein Composite Nanoparticles†

doi:10.7503/cjcu20150115

Abstract

Abstract:

In order to protect the physiological activity of resveratrol, resveratrol-hordein nanoparticles were self-assembled by liquid-liquid dispersion method. The mean diameter of the nanoparticles[(135.0±2.5) nm] was measured with dynamic light scattering method, with encapsulation efficiency of resveratrol(90.4%) and drug loading(18.8%) were obtained by ultraviolet spectrophotometry method. Scanning electron microscope(SEM) showed that the morphology of nanoparticles was smooth and spherical. The effect of composite nanoparticles carrier type on the light stability of resvertrol was investigated. The results showed that the stability of resveratrol in nanoparticles was improved by 26% compared with that of free resveratrol after ultraviolet irradiation for 18 h. Resveratrol release behavior was studied in the simulated gastro-intestinal tract. The data revealed that 35% resveratrol was released from resveratrol-hordein nanoparticles in simulated gastric fluid at the first 2 h, and then released slowly, until 56% resveratrol was released after 6 h. While in the simulated intestinal fluid, 42% resveratrol was released in the first 2 h, and then steadily released from nanoparticles according to zero-order release kinetics during the first 4 h. After 6 h, 88% resveratrol was released. The antioxidant capability of free resvertrol and composite nanoparticles was studied. 1,1-Diphenyl-2-picrylhydrazyl radical(DPPH) experiment results showed that resvertrol in composite nanoparticles had a little more scavenging effect on DPPH free radicals(IC₅₀=0.4182 mmol/L) compared with free resvertrol(IC₅₀=0.4378 mmol/L). A HepG2 cellular antioxidant activity(CAA) assay further demonstrated the above results.

Key words: Resveratrol, Hordein, Nanoparticle, Self-assembly

CLC Number:

O625
TS201

TrendMD:

GUAN Xiao, YIN Ting, HAN Fei. Light Stability, Controlled-release and Antioxidation of Resveratrol-hordein Composite Nanoparticles^†[J]. Chem. J. Chinese Universities, 2015, 36(9): 1707.

Figures/Tables 10

Fig.1 SEM image of resveratrol-hordein nanoparticles

Fig.2 FTIR spectra of resveratrol(a), hordein nanoparticles(b) and resveratrol-hordein nanoparticles(c)

Fig.3 Stability curve of resveratrol(a) and resveratrol-hordein nanoparticle(b) under UV-light exposure

Fig.4 Release profile of resveratrol in simulated gastric(a) and intestinal fluid(b)

Fig.5 Absorbance curves of DPPH under the conditions of coexistence with resveratrol(A) and resveratrol-hordein nanoparticles(B)

Fig.6 DPPH scavenging standard curves of resveratrol(a) and resveratrol-hordein nanoparticle(b)

Fig.7 Peroxyl radical-induced oxidation of DCFH to DCF in HepG2 cells and the inhibition of oxidation(A) Resveratrol; (B) resveratrol-hordein nanoparticles; (C) quercetin standard.c(Resveratrol)/(μmol·L-1): a. 0; b. 1; c. 5; d. 10; e. 20; f. 30; g. 40.

Fig.8 Dose-response curve for inhibition of peroxyl radical-induced DCFH oxidation(A) Resveratrol; (B) resveratrol-hordein nanoparticles; (C) quercetin standard.

Fig.9 Median effect plots for inhibition of peroxyl radical-induced DCFH oxidation(A) Resveratrol; (B) resveratrol-hordein nanoparticles; (C) quercetin standard.

Table 1 Comparison of cellular antioxidant activity between revertrol and revertrol-hordein nanoparticle

Antioxidant	EC₅₀/(μmol·L^-1)	CAA/[n(QE)/100 $n c *$ ]
Quercetin	6.41	100
Resveratrol	48.46	13.22
Resveratrol-hordein nanoparticles	45.31	14.14

Table 1 Comparison of cellular antioxidant activity between revertrol and revertrol-hordein nanoparticle

Antioxidant	EC₅₀/(μmol·L^-1)	CAA/[n(QE)/100 $n c *$ ]
Quercetin	6.41	100
Resveratrol	48.46	13.22
Resveratrol-hordein nanoparticles	45.31	14.14

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