Simultaneous Determination of Multi-veterinary Drug Residues in Honey by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry and Multiwalled Carbon Nanotubes Cleaning

doi:10.7503/cjcu20130764

Abstract

Abstract:

A method for determining multi-veterinary drug residues in honey by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) and multiwalled carbon nanotubes cleaning was established. This method enabled the comprehensive screening for 52 drugs including sulfonamides, quinolones, nitroimidazoles and tetracyclines. The samples were diluted by Na₂EDTA-Mcllvaine solution(pH=4), the clean-up and proconcentration of the analytes were carried out by solid phase extraction cartridge packed with multi-walled carbon nanotubes(MWCNTs). The target analytes were separated by Waters C₁₈ column with gradient elution using acetonitrile and 0.1%(volum fraction) formate in water as mobile phases. The analytes were detected by tandem quadrupole mass spectrometry after positive electrospray ionization by multiple reaction monitoring(MRM). External standard method with matrix was used to determine the results. The correlation coefficient was greater than 0.99 for each drugs. The limits of quantitation(LOQ) were 1.5—7.5 μg/kg. The mean recoveries at the three spiked levels of 7.5, 25, 50 μg/kg were 67.3%—117.8%. The relative standard deviation(RSD) were 1.9%—17.4%. The results indicated that using multi-walled carbon nanotubes as solid phase extraction adsorbent was efficient for the purification. The simplicity, sensitivity and good precision of the method made it be well suitable for determination of multi-veterinary drug residues in honey.

Key words: Multiwalled carbon nanotubes(MWCNTs), Solid phase extraction, Ultra performance liquid chromatography-tandem mass spectrometry, Residue

CLC Number:

O657.63

TrendMD:

CAO Hui, CHEN Xiao-Zhen, ZHU Yan, LI Zu-Guang. Simultaneous Determination of Multi-veterinary Drug Residues in Honey by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry and Multiwalled Carbon Nanotubes Cleaning[J]. Chem. J. Chinese Universities, 2013, 34(12): 2710.

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