Study on Apoptosis Process of CaSki via Fast Line-scanning Raman Imaging†

doi:10.7503/cjcu20160265

Abstract

Abstract:

Cisplatin-induced apoptosis of CaSki cell was studied via Raman imaging technique. Fast line-scanning Raman imaging method could reveal the high temporal and spatial information of biomolecules including cytochrome c, protein and lipid during the apoptosis process. The results indicate that the relative intensity of cytochrome c to protein(I₇₅₀/I₁₆₆₀) could reflect the stage of apoptosis. Compared with the traditional methylthiazoletetrazolium(MTT) method, Raman imaging could investigate the apoptosis of single living cell at a much higher temporal resolution and provide the molecular fingerprint information for revealing the cell-drug interaction.

Key words: Cervical cancer, Cytochrome c, Apoptosis, Raman spectroscopy, Raman imaging, Methylthiazoletetrazolium(MTT) method

CLC Number:

O657.37
O644

TrendMD:

ZHANG Jinliang, MA Xin, XU Mengxi, ZONG Cheng, REN Bin. Study on Apoptosis Process of CaSki via Fast Line-scanning Raman Imaging^†[J]. Chem. J. Chinese Universities, 2016, 37(7): 1257.

Figures/Tables 4

Fig.1 Viability of CaSki cells after being treated with different concentrations of cisplatin for 24 h

Fig.2 Time-depended viability of CaSki cells after being treated with 4 mmol/L cisplatin

Fig.3 Time depended bright field images(A1—F1) and Raman images using raman peaks of cytochrome c(750 cm-1)(A2—F2), protein(1660 cm-1)(A3—F3), and lipid(2852 cm-1)(A4—F4) of cells after being treated with 4 mmol/L cisplatin for 0 h(A1—A4), 4 h(B1—B4), 8 h(C1—C4), 12 h(D1—D4), 24 h(E1—E4) and 48 h(F1—F4)

Fig.4 Intensity of 750 and 1660 cm-1 peaks and ratio of I750/I1660 of the cells after being treated with 4 mmol/L cisplatin for different time

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