Chem. J. Chinese Universities ›› 2020, Vol. 41 ›› Issue (7): 1521.doi: 10.7503/cjcu20200129

• Analytical Chemistry • Previous Articles     Next Articles

Preparation and Application of Melamine Imprinted Material Using Itaconic Acid as Multidentate Functional Monomer

WANG Xiaoru1,ZHANG Na1,2,XING Jun1,*()   

  1. 1. College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China
    2. Researcher of Analytical Technology Development Unit, Hec Pharm Co. Ltd., Yidu 443300, China
  • Received:2020-03-09 Online:2020-07-10 Published:2020-04-28
  • Contact: XING Jun E-mail:gc-ms@263.net

Abstract:

A molecularly imprinted polymer(MIP) was prepared using melamine(MEL) as template, itaconic acid(IA) as functional monomer and ethylene glycol dimethacrylate(EGDMA) as cross-linker. The effect of molar ratio among these components on the selectivity of MIP was investigated. It is found that the prepared MIP presents the best selectivity to MEL when molar ratio of MEL to IA is 1:1.5. The selectivity coefficient(k) of the synthesized MIP to MEL is 10.41(CYA as control). Since the molar ratio of carboxyl groups(recognition sites) of IA and amino groups(recognizable groups) of MEL is exactly 1: 1, the non-specific adsorption caused by excessive functional monomers will be avoided. Scatchard analysis indicates that MIP only exhibits specific binding to MEL within the concentration range from 1 to 100 mg/L of MEL. On the other hand, when CYR is used as the control, the kNIP is less than 1 but the kMIP is greater than 1. This result implies the synthesized MIP has good discriminating ability for the shape of molecules. Using the prepared MIP as the adsorbent of solid phase extraction(SPE), a method of MISPE hyphenated to high performance liquid chromatography with UV detector(MISPE-HPLC/UV) was established for determination of melamine. The recoveries of MEL in the spiked milk and milk powder samples vary from 92.34% to 109.4%.

Key words: Molecular imprinting, Multidentate functional monomer, Itaconic acid, Melamine, Solid phase extraction

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