NMR Studies of Gd(DTPA-BIN)in Human Serum Albumin Solution

Abstract

Abstract: Stable gadolinium complexes, such as Gd(DTPA) and Gd(DOTA), are usually used as the contrast agents for magnetic resonance imaging(MRI). Reported here are the enhanced relaxation properties of a novel gadolinium complex, diethylene triaminopentaacetate bis(isoniazid) Gd(DTPA BIN), in aqueous and in human serum albumin(HSA) solution, which indicates that (1) two Gd(DTPA BIN) can integrate non covalently with one HSAwith an equilibrium constant of 0.02 mmol^-2 ·L²; (2) the relaxivities are 3.28 and 4.92 mmol^-1 ·L·s^-1 for the free Gd(DTPA BIN) and the Gd(DTPA BIN)₂ HSAconjugator, respectively; (3) the rotational correlation time of protein conjugator is notably higher than that of the free complex. The above results may imply that Gd(DTPA BIN) has a higher tissue selectivity than that of its parent Gd(DTPA).

Key words: Gd (DTPA-BIN), Human serum albumin, Relaxation analysis

CLC Number:

O615.5

TrendMD:

LI Xin-Yu, ZHANG Shan-Rong, JING Feng-Ying, WU Yi-Jie, PEI Feng-Kui . NMR Studies of Gd(DTPA-BIN)in Human Serum Albumin Solution[J]. Chem. J. Chinese Universities, 1998, 19(12): 2026.

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NMR Studies of Gd(DTPA-BIN)in Human Serum Albumin Solution

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