2D NMR Studies of Chinese Rape Pollen Dodecapeptide

Abstract

Abstract: The dodecapeptide(RPP) is an active peptide,which we extracted from chinese rape pollen.The dodecapeptide enhances the activity of TNFon murine tumor cells and also shows immunopromotive activities.The possible conformation of the RPP,which has been previously studied by circular dichroism,has been studied by one and two dimensional NMR spectroscopy.All resonance assignments were made by use of two dimensional correlation spectroscopy.The problem of sequential resonance assignments was solved by using two dimensional NOE spectroscopy.The dihedral angles of the amide planes of the RPP,were calculated from the measured coupling constants using Karplus equation.The temperature coefficient and NOEin different solvents together suggest that RPP exists mainly as random coil in aqueous solution and some sections in the RPPexist as γ-turn.

Key words: Pollen, Peptide, 2D NMR

TrendMD:

SONG Yan-Ling, GAO Xin-Feng, WAN Kai-Ke, SUN Hong-Yin, JIN Sheng. 2D NMR Studies of Chinese Rape Pollen Dodecapeptide[J]. Chem. J. Chinese Universities, 1996, 17(10): 1550.

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2D NMR Studies of Chinese Rape Pollen Dodecapeptide

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