Structural Characterization of Peptide Calcium Chelate VGLPNSR-Ca and Its Calcium Absorption Ability in Caco-2 Cell Monolayer

doi:10.7503/cjcu20190064

Chem. J. Chinese Universities ›› 2019, Vol. 40 ›› Issue (8): 1643.doi: 10.7503/cjcu20190064

• Organic Chemistry • Previous Articles Next Articles

Structural Characterization of Peptide Calcium Chelate VGLPNSR-Ca and Its Calcium Absorption Ability in Caco-2 Cell Monolayer

LIU Bingtong, ZHUANG Yongliang

Yunnan Institute of Food Safety, Kunming University of Science and Technology, Kunming 650500, China

Received:2019-01-23 Revised:2019-07-23 Online:2019-08-10 Published:2019-08-02
Supported by:
Supported by the National Natural Science Foundation of China(No. 31360381).

Abstract

Abstract: The peptide calcium chelate VGLPNSR-Ca was prepared from Val-Gly-Leu-Pro-Asn-Ser-Arg(VGLPNSR) and calcium chloride. The structure of VGLPNSR-Ca was analyzed by means of UV spectroscopy, X-ray diffraction(XRD), scanning electron microscopy(SEM), Fourier tranform infrared spectroscopy(FTIR) and mass spectrometry. The calcium absorbing ability of VGLPNSR-Ca in the intestine was studied by Caco-2 cell monolayer model in vitro. According to the results of UV, XRD and SEM, VGLPNSR-Ca was formed as a stable compound. FTIR analysis indicated that the chelation sites of VGLPNSR-Ca were mainly the nitrogen atom of the amino group and the oxygen atom of the carboxyl group. Combined with LC-MS/MS, the chelation reaction of VGLPNSR with calcium ions was mainly involved in the nitrogen terminal of Val, the carbon terminal of Arg and the amino acid Asn. Based on the above results and analyses, the molecular structure of VGLPNSR-Ca was predicted. In addition, the calcium absorption rate of VGLPNSR-Ca in Caco-2 cell monolayer was 31.97%, twice as high as calcium absorption of calcium chloride, proving that VGLPNSR-Ca can effectively improve the bioavailability of calcium.

Key words: Peptide calcium chelate, Calcium binding capacity, Structural characteristics, Caco-2 cell, Calcium absorption characteristics

CLC Number:

O629.72

TrendMD:

LIU Bingtong, ZHUANG Yongliang. Structural Characterization of Peptide Calcium Chelate VGLPNSR-Ca and Its Calcium Absorption Ability in Caco-2 Cell Monolayer[J]. Chem. J. Chinese Universities, 2019, 40(8): 1643.

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Structural Characterization of Peptide Calcium Chelate VGLPNSR-Ca and Its Calcium Absorption Ability in Caco-2 Cell Monolayer

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