Binding Effect of Tb3+and Ca2+Ions on Trichosanthin

Chem. J. Chinese Universities ›› 1995, Vol. 16 ›› Issue (12): 1907.

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Binding Effect of Tb³⁺and Ca²⁺Ions on Trichosanthin

LIU Guo-Hong¹, LI Wen-Lian³, HAO Qian¹, ZHAO Xu³, ZHANG Yan¹, YANG Hong¹, HUANG Zhong-Li², GAO Gui², DONG Qing-Chu¹, LI Qing-Shan¹

1. Department of Molecular Biology , Jilin University, Changchun, 130023;
2. National Laboratory of Enzyme Engineering, Jilin University, Changchun, 130023;
3. Changchun Institute of Physics, Chinese Academy of Sciences

Received:1994-12-30 Revised:1995-08-21 Online:1995-12-24 Published:1995-12-24

Abstract

Abstract: Tb³⁺has been used as a fluorescent probe to study the structure of trichosanthin(TCS).The nonradioactive energy transfer from fluorescent active tryptophan(Trp)residue to the bound Tb³⁺ions take place and the fluorescence of Trp is decreased when Tb³⁺ions are bound to TCSto replace the Ca²⁺in it.According to the results of Trp fluorescence and the information given from CDand UVabsorption spectra,we have found that the affinity of Tb³⁺to TCS is lower than that of Ca²⁺ions to TCS. Significant change of TCS structure was not observed with the Tb³⁺ions binding,The microenvironment surrounded Trp and Tyr may be changed slightly with the Tb³⁺binding.The conformation of Ca²⁺-free TCS was significantly changed compared with that of native TCS with the Trp residue moving into the interior of TCS molecules。 The Ca²⁺-binding site in TCS can be maintained partly when the Ca²⁺is removed from TCSmolecules.

Key words: Tb³⁺,Ca²⁺, Trichosanthin, Fluorescent probe

TrendMD:

LIU Guo-Hong, LI Wen-Lian, HAO Qian, ZHAO Xu, ZHANG Yan, YANG Hong, HUANG Zhong-Li, GAO Gui, DONG Qing-Chu, LI Qing-Shan. Binding Effect of Tb³⁺and Ca²⁺Ions on Trichosanthin[J]. Chem. J. Chinese Universities, 1995, 16(12): 1907.

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Binding Effect of Tb³⁺and Ca²⁺Ions on Trichosanthin

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