Chem. J. Chinese Universities ›› 1994, Vol. 15 ›› Issue (2): 189.

• Articles • Previous Articles     Next Articles

A Study of Ascorbic Acid Sensor Based on Ascorbic Oxidase Immobilized in an Electropolymerized Poly(o-phenylenediamine) Film

JIN Li-Tong, ZHAO Gui-Zhu, FANG Yu-Zhi   

  1. Department of Chemistry, East China Normal University, Shanghai, 200062
  • Received:1993-04-10 Revised:1993-10-15 Online:1994-02-24 Published:1994-02-24

Abstract: A chemically modified electrode biosensor is reported.The biosensor has been applied to determine ascorbic acid in human serum.The linear range of detection is 1.0×10-5-2.5×10-7mol/L and detection limit is 1.0×10-8mol/L.The response time is 7 s.The biosensor has advantages of high selectivity,sensitivity and short response time.

Key words: Biosensor, Ascorbate oxidase, Enzyme electrode, o-Phenylenediamine, Modified electrode

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