Preparation of Carbon Dots Fluorescent Marker and Its Application in Highly Selective NO2‒ Detection

doi:10.7503/cjcu20240519

Abstract

Abstract:

In this work， the yellow-green carbon dots（λ_ex=440 nm） with a fluorescence emission wavelength of 535 nm were prepared by a one-step hydrothermal method using dopamine hydrochloride and o-phenylenediamine as raw materials， and then used as fluorescent marker materials for NO₂^‒ detection in water samples after purified and separated. The size of the carbon dots is about 3 nm with good dispersion， excellent salt resistance， stable fluorescence under a broad pH value. However， nitrite contaminants in water sources can cause methemoglobinemia disease and has potential carcinogenic risk. The results of the selectivity of the yellow-green carbon dots prepared to detect common contaminants in water samples showed that the carbon dots had a highly specific response to nitrite， and the detection limit was 0.1166 μg/mL（S/N=3， n=3）， which meets the requirements of Chinese national standards for determination of NO₂^‒ in drinking water（≤1 μg/mL）. The detection range of 0—60 μg/mL fits the Stern-Volmer equation， and the range of 0—10 μg/mL（y=0.02x+0.000876， R²=0.9868） and 12—60 μg/mL（y=0.011x+0.198， R²=0.9937） have good linear responses. In addition， the recoveries of three spiked water samples are from 90.8% to 100.8%（RSD=0.03%—0.86%， n=3） for the validation test. The method of the fluorescent carbon dots marker for contaminants developed in this work is fast and simple， low-cost and has greatly potential in trace nitrite analysis and can be an effective supplementary method for the monitoring of drinking water sources quality.

Key words: One-step hydrothermal method, Carbon dots, NO₂^?, Water quality monitoring

CLC Number:

O657.3

TrendMD:

WANG Changying, ZHANG Dawei, CHEN Guanji, ZHANG Zhenwei, XIAO Weihong, WANG Bin, CHEN Qidan, YANG Bai. Preparation of Carbon Dots Fluorescent Marker and Its Application in Highly Selective NO₂^‒ Detection[J]. Chem. J. Chinese Universities, 2025, 46(6): 20240519.

Figures/Tables 7

References 25

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Sample	Spiked/（μg·mL^‒1）	Detected/（μg·mL^‒1）	Recovery（%）	RSD（%， n=3）
1	1	1.0079	100.8	0.44
	6	5.7063	95.1	0.29
	10	9.6881	96.9	0.34
2	1	0.9901	99.0	0.62
	6	5.6361	93.9	0.03
	10	9.6372	96.4	0.81
3	1	0.9081	90.8	0.86
	6	5.8335	97.2	0.22
	10	9.1567	91.6	0.63

Sample	Spiked/（μg·mL^‒1）	Detected/（μg·mL^‒1）	Recovery（%）	RSD（%， n=3）
1	1	1.0079	100.8	0.44
	6	5.7063	95.1	0.29
	10	9.6881	96.9	0.34
2	1	0.9901	99.0	0.62
	6	5.6361	93.9	0.03
	10	9.6372	96.4	0.81
3	1	0.9081	90.8	0.86
	6	5.8335	97.2	0.22
	10	9.1567	91.6	0.63

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Preparation of Carbon Dots Fluorescent Marker and Its Application in Highly Selective NO₂^‒ Detection

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