Abstract: Cell derived fluorescent carbon points (CDs) were synthesized via one step hydrothermal method. It is found that the CDs have excellent peroxide-like catalytic activity, which can catalyze the hydrogen peroxide to produce hydroxyl radical. Based on this, the hydrogen peroxide produced by glucose oxidation and CDs could consecutively oxidize p-phenylenediamine (PPD to produce PPDox (2,5-Diamino-N,N'-di-(4-aminophenyl)-2,5-cyclohexadiene-1,4-diimine). By virtue of the inner filter effect of PPDox on CDs, a dual-mode colorimetric and fluorescent detection of glucose was establised. The limit of detection of the present assay is 41 μmol/L and 13 μmol/L for the colorimetric method and the fluorescence method, respectively. In this work, CDs can not only be used as a peroxide-like enzyme to catalyze hydrogen peroxide to produce hydroxyl radical for PPD oxidation, but also be used as a fluorescent indicator to indicate the change of glucose concentration through the internal filtration effect.

Key words: Cell derived fluorescent carbon dots, Peroxide-like, Glucose probe