Chem. J. Chinese Universities ›› 2025, Vol. 46 ›› Issue (6): 20240386.doi: 10.7503/cjcu20240386

• Article • Previous Articles     Next Articles

Colorimetric and Fluorescence Determination of Glucose Based on Cell-derived Fluorescent Carbon Dots

LIU Yingqi1, WANG Yemei1, JIANG Kai1, ZHENG Fenfen1(), ZHU Junjie2()   

  1. 1.School of Environmental & Chemical Engineering,Jiangsu University of Science and Technology,Zhenjiang 212003,China
    2.State Key Laboratory of Analytical for Life Science,School of Chemistry and Chemical Engineering,Nanjing University,Nanjing 210023,China
  • Received:2024-08-15 Online:2025-06-10 Published:2024-09-20
  • Contact: ZHENG Fenfen, ZHU Junjie E-mail:zhengfenfen@just.edu.cn;jjzhu@nju.edu.cn
  • Supported by:
    the National Natural Science Foundation of China(21804059);the Postgraduate Research & Practice Innovation Program of Jiangsu Province, China(KYCX23_3898)

Abstract:

Cell derived fluorescent carbon points(CDs) were synthesized via one step hydrothermal method. It is found that the CDs have excellent peroxide-like catalytic activity, which can catalyze the hydrogen peroxide to produce hydroxyl radical. Based on this, the hydrogen peroxide produced by glucose oxidation and CDs could consecutively oxidize p-phenylenediamine(PPD) to produce PPDox[2,5-Diamino-NN′-di-(4-aminophenyl)-2,5-cyclohexadiene-1,4-diimine]. By virtue of the inner filer effect of PPDox on CDs, a dual-mode colorimetric and fluorescent detection of glucose was establised. The limit of detection of the present assay are 41 µmol/L and 13 µmol/L for the colorimetric method and the fluorescence method, respectively. In this work, CDs can not only be used as a peroxide-like enzyme to catalyze hydrogen peroxide to produce hydroxyl radical for PPD oxidation, but also be used as a fluorescent indicator to indicate the change of glucose concentration through the internal filtration effect.

Key words: Cell derived fluorescent carbon dots, Peroxide-like, Glucose probe

CLC Number: 

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