Fabrication and Performance for Fe3O4 Nanoparticles Surface Grafted Poly(γ-benzyl-L-glutamate) Porous Microcarriers†

doi:10.7503/cjcu20160701

Abstract

Abstract:

Ferroferric oxide/poly(γ-benzyl-L-glutamate)(PBLG) composite materials(Fe₃O₄-g-PBLG) was synthesized by initiating γ-benzyl-L-glutamate-N-carboxyanhydride(BLG-NCA) polymerization on the surface of amino-functionalized ferroferric oxide(Fe₃O₄) nanoparticles. Fe₃O₄-g-PBLG composites was confirmed by Fourier transform infrared and X-ray diffraction. Thermogravimetric(TGA) study showed that the grafting ratios of Fe₃O₄-g-PBLG were 66.36%, 79.66%, and 89.52%, and grafting ratio of Fe₃O₄-g-PBLG composite could be controlled. The Fe₃O₄-g-PBLG with grafting ratio of 89.52% was used to fabricate magnetic Fe₃O₄-g-PBLG porous microcarriers. The porous microcarriers possessed suitable density(1.034 g/mL), porosity(92.57%), particle size(200—300 μm), pore size(40—50 μm) and water retention(370%—400%). Meanwhile, microcarriers exhibited superparamagnetism. Under the action of magnetic fields, the microcarriers can be arranged into any shape, showing significant advantages for the treatment of complex structure of the bone defects. These results showed that Fe₃O₄-g-PBLG porous microcarriers might be applied in bone tissue engineering.

Key words: Fe3O4, Poly(-benzyl-L-glutamate)(PBLG), Porous microcarrier, Magnetic property, Bone tissue engineering

CLC Number:

O636

TrendMD:

XU Shenghua, XIA Pengfei, ZHANG Kunxi, GAO Long, YIN Jingbo. Fabrication and Performance for Fe₃O₄ Nanoparticles Surface Grafted Poly(γ-benzyl-L-glutamate) Porous Microcarriers^†[J]. Chem. J. Chinese Universities, 2017, 38(7): 1295.

Figures/Tables 10

Scheme 2 Fabrication of Fe3O4-g-PBLG composites and its porous microcarriers[18]

Fig.1 AFM images of Fe3O4 nano-particles(A), Fe3O4-g-PBLG composite material(B) and 3D structure of Fe3O4-g-PBLG composite material(C)

Fig.2 Diameter of Fe3O4 nano-particles(a) and Fe3O4-g-PBLG composite material(b)

Fig.3 FTIR spectra(A) and XRD patterns(B) of Fe3O4 nano-particles(a), Fe3O4-g-PBLG composite with grafting ratio of 70%(b), 80%(c) and 90%(d) and PBLG(e)

Fig.4 TGA(A) and DSC(B) curves of Fe3O4 nano-particles(a), Fe3O4-g-PBLG composite with grafting ratios of 70%(b), 80%(c) and 90%(d) and PBLG(e)

Fig.5 SEM images of Fe3O4-g-PBLG porous miccarriers with grafting ratios of 70%(A, A'), 80%(B, B') and 90%(C, C')

Fig.6 Porosity and density(A) and water retenticity(B) of Fe3O4-g-PBLG porous microcarriers with different grafting ratios

Fig.7 Hysteresis regression line of Fe3O4 nanoparticles(a) and Fe3O4-g-PBLG porous microcarriers with grafting ratio of 90%(b)

Fig.8 Arrayed behavior of Fe3O4-g-PBLG porous microcarriers with grafting ratio of 90% under different kinds of magnetic field

Fig.9 Confocal laser scanning microscopy images of cell adhesion in Fe3O4-g-PBLG porous microcarriers with grafting ratio of 90%(A) 3D image. Scanning depths in each microcarrier: (B) 1/10 diameter in depth; (C) 2/10 diameter in depth;(D) 3/10 diameter in depth; (E) 4/10 diameter in depth; (F) 5/10 diameter in depth.

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Fabrication and Performance for Fe₃O₄ Nanoparticles Surface Grafted Poly(γ-benzyl-L-glutamate) Porous Microcarriers^†

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