Preparation of Gold Nanoflower-double Layer Silica Core-shell Nanoparticles and Their Photothermal Properties and Metabolism in vivo†

doi:10.7503/cjcu20180299

Abstract

Abstract:

Gold nanoflower-double layer silica core-shell nanoparticles were prepared by a seed-mediated growth strategy and a modified Stöber method. The photothermal properties and the biotoxicity in vivo of the as-prepared core-shell nanoparticles were studied. The nude mice bearing oral cancer cell CAL27 were taken as the animal model to investigate the distribution, metabolism and passive targeting enrichment of the core-shell nanoparticles in vivo by the photoacoustic imaging technique. The results showed that the as-prepared core-shell nanoparticles exhibited excellent photothermal conversion properties in near infrared(NIR) region. The core-shell nanoparticles could be well metabolized in the liver, and could be excreted outside of the body through the intestinal tract. More importantly, the core-shell nanoparticles exhibited passive targeting enrichment in the tumor tissue through EPR effect. Our study show that the gold nanoflower-double layer silica core-shell nanoparticles would be an ideal photoacoustic imaging agent and a NIR photothermal therapy agent for diagnose and therapy of malignant tumors such as oral cancer.

Key words: Gold nanoflower-silica nanocomposites, Core-shell nanoperticles, Photoacustic imaging(PAI), Near infrared(NIR) photothermal conversion, Oral cancer

CLC Number:

O614
O644

TrendMD:

SONG Wenzhi,LI Hui,ZHANG Yan,HE Dan,LI Yingzi,HUANG Zhenzhen,LIU Xin,YIN Wanzhong. Preparation of Gold Nanoflower-double Layer Silica Core-shell Nanoparticles and Their Photothermal Properties and Metabolism in vivo^†[J]. Chem. J. Chinese Universities, 2018, 39(12): 2644.

Figures/Tables 6

Scheme 1 Synthesis of AuNF@SiO2@mSiO2 nanoparticles

Fig.1 TEM image(A) and UV-Vis spectrum(B) of the AuNF@SiO2@mSiO2 nanoparticles

Fig.2 Photo-thermal conversion curves of the aqueous dispersions of AuNF@SiO2@mSiO2 nanoparticles with different concentrations under the NIR laser irradiation with power of 5 W/cm2(A), 7 W/cm2(B) and 9 W/cm2(C)a. Control; b. 50 μg/mL; c. 100 μg/mL; d. 150 μg/mL; e. 200 μg/mL; f. 250 μg/mL.

Fig.3 Morphological observation on different organs of the nude mice(A1—D1): control group; (A2—D2): test group. (A1, A2): liver; (B1, B2): heart; (C1, C2): kidney; (D1, D2): spleen.

Table 1 Serum biochemical indices of the nude mice injected with AuNF@SiO2@mSiO2 nanoparticles

Group	c(BUN)/ (μmol·L^-1)	c(Cr)/ (μmol·L^-1)	c(GPT)/ (U·L^-1)	c(GOT)/ (U·L^-1)	c(CK)/ (U·mL^-1)	c(CK-MB)/ (U·L^-1)	c(LDH)/ (U·L^-1)
Experimental	2.121±0.066	55.975±1.234	110.245±3.075	205.989±6.022	0.911±0.058	2010.113±78.94	893.717±20.256
Control	2.158±0.091	54.70±1.264	108.843±2.587	201.655±4.578	0.877±0.041	1962.32±63.825	882.432±23.278

Fig.4 Photoacoustic imaging of the living CAL27-tumour-bearing mice after tail intravenous injection of AuNF@SiO2@mSiO2 nanoparticles

References 37

[1]	Torre L.A., Bray F., Siegel R.L., Ferlay J., Lortet-Tieulent J., Jemal A., CA Cancer J.Clin., 2015, 65(2), 87—108
[2]	Jain P.K., Lee K.S., El-Sayed I.H., El-Sayed M. A.J., Phys. Chem.B,2006, 110(14), 7238—7248
[3]	Saverot S.E., Reese L.M., Cimini D., Vikesland P.J., Bickford L.R., Nanoscale Res. Lett.,2015, 10, 241—254
[4]	Fay B.L., Melamed J.R., Day E.S., Int. J. Nanomedicine,2015, 10, 6931—6941
[5]	Song W.Z., Jiang Y.P., Ji X.H., Zhao L.L., Yin W.Z., Yang W.S., Chem. J. Chinese Universities,2012, 33(9), 1886—1888
	(宋文植, 姜雅萍, 纪小会, 赵丽丽, 尹万忠, 杨文胜. 高等学校化学学报, 2012, 33(9), 1886—1888)
[6]	Huang H., Yang D.P., Liu M.H., Wang X.S., Zhang Z.Y., Zhou G.D., Liu W., Cao Y.L., Zhang W.J., Wang X.S., Int. J. Nanomedicine,2017, 12, 2829—2843
[7]	Gobin A.M., Moon J.J., West J.L., Int. J. Nanomedicine,2008, 3(3), 351—358
[8]	Manivasagan P., Bharathiraja S., Moorthy M.S., Oh Y.O., Song K., Seo H., Oh J., ACS Appl. Mater. Interfaces,2017, 9(17), 14633—14646
[9]	Rejiya C.S., Jatish K., Raji V., Vibin M., Annie A., Pharmacol. Res.,2012, 65(2), 261—269
[10]	Huang X., El-Sayed I.H., Qian W., El-Sayed M. A., J. Am. Chem. Soc., 2006, 128, 2115—2120
[11]	Jiao P., Otto M., Geng Q., Li C., Li F., Butch E.R., Snyder S.E., Zhou H., Yan B., J.Mater.Chem., 2016, 4(3), 513—520
[12]	Lin A.Y., Young J.K., Nixon A.V., Drezek R.A., Small,2014, 10(16), 3246—3251
[13]	Luo T., Huang P., Gao G., Shen G., Fu S., Cui D., Zhou C., Ren Q., Opt. Express,2011, 19(18), 17030—17039
[14]	Yi S.J., Sun L.M., Lenaghan S.C., Wang Y.Z., Chong X.Y., Zhang Z.L., Zhang M.J., RSC Adv.,2013, 3(26), 10139—10144
[15]	Chen J., Sheng Z., Li P., Wu M., Zhang N., Yu X.F., Wang Y., Hu D., Zheng H., Wang G.P., Nanoscale,2017, 9(33), 11888—11901
[16]	Zhou G., Xiao H., Li X., Huang Y., Song W., Song L., Chen M., Cheng D., Shuai X., Acta Biomater.,2017, 64, 223—236
[17]	Sun Y., Wang Q., Chen J., Liu L., Ding L., Shen M Li J., Han B., Duan Y., Theranostics,2017, 7(18), 4424—4444
[18]	Sun Q., You Q., Pang X., Tan X., Wang J., Liu L., Guo F., Tan F., Li N., Biomaterials,2017, 122, 188—200
[19]	Cheng B., He H., Huang T., Berr S.S., He J., Fan D., Zhang J., Xu P., J. Biomed. Nanotechno.,2016, 12(3), 435—449
[20]	Huang Y., Rosei F., Vetrone F., Nanoscale,2015, 7(12), 5178—5185
[21]	Stassi S., Cauda V., Canavese G., Manfredi D., Pirri C.F., Eur. J. Inorg. Chem.,2012, 16, 2669—2673
[22]	Haiss W., Thanh N. T.K., Aveyard J., Fernig D.G., Anal. Chem.,2007, 79(11), 4215—4221
[23]	Stöber W., Fink A., Bohn E., J. Colloid Interface Sci.,1968, 26(1), 62—69
[24]	Yang J.P., Zhang F., Chen Y.R., Qian S., Hu P., Li W., Deng Y.H., Fang Y., Han L., Mohammad L., Zhao D.Y., Chem. Commun.,2011, 47(42), 11618—11620
[25]	Lai C.Y., Trewyn B.G., Jeftinija D.M., Jeftinija K., Xu S., Jeftinija S., Lin V.S., J. Am. Chem. Soc.,2003, 125(15), 4451—4459
[26]	Feng J., Wang Z., Shen B., Zhang L., Yang X., He N., RSC Adv.,2014, 4(54), 28683—28690
[27]	Austin L.A., Mackey M.A., Dreaden E.C., Elsayed M.A., Arch. Toxicol.,2014, 88(7), 1391—1417
[28]	Li W., Chen X., Nanomedicine,2015, 10(2), 299—320
[29]	Xu M., Wang L.V., Rev. Sci. Instrum.,2006, 77, 041101
[30]	Andreev V.G., Karabutov A.A., OraevskyA. A., IEEE Trans. Ultrason. Ferroelectr. Freq. Control,2003, 50(10), 1383—1390
[31]	Wang L.V., Nat. Photonics, 2009, 3(9), 503—509
[32]	Erpelding T.N., Kim C., Pramanik M., Jankovic L., Maslov K., Guo Z., Margenthaler J.A., Pashley M.D., Wang L.V., Radiology,2010, 256(1), 102—110
[33]	Kim C., Cho E.C., Chen J., Song K.H., Au L., Favazza C., Zhang Q., Cobley C.M., Gao F., Xia Y., Wang L.V., ACS Nano,2010, 4(8), 4559—4564
[34]	Emelianov S.Y., Li P.C., O’Donnell M., Phys. Today,2009, 62(8), 34—39
[35]	Zhang E.Z., Povazay B., Laufer J., Alex A., Hofer B., Pedley B., Glittenberg C., Treeby B., Cox B., Beard P., Drexler W., Biomed. Opt. Express,2011, 2(8), 2202—2215
[36]	Jansen K., van der Steen A. F. W., van Beusekom H. M. M., Oosterhuis J.W., van Soest G., Opt. Lett.,2011, 36(5), 597—599
[37]	Maeda H., Nakamura H., Fang J., Adv. Drug Deliv. Rev.,2013, 65(1), 71—79