Screening of α-Glucosidase Inhibitors in Ginkgo Biloba Extract Using Ultrafiltration LC-ESI-MSn

doi:10.7503/cjcu20121098

Abstract

Abstract:

In vitro α-glucosidase inhibition assays and ultrafiltration liquid chromatography coupled to electrospray ionization tandem mass spectrometry(ultrafiltration LC-ESI-MSⁿ) were combined to screen α-glucosidase inhibitors from ginkgo biloba extract. The enzyme inhibition studies were carried out spectrophotometrically in a 96-well microplate reader. Using 4-nitrophenyl α-D-glucopyranoside(PNPG) as the substrate and acarbose as a positive control, α-glucosidase inhibitory activity of the five kinds of Chinese herb extracts which rich in flavonoids were determined, the results showed that the ginkgo biloba extract has the strongest the α-glucosidase inhibitory activity. Ultrafiltration LC-ESI-MSⁿ was used to screen and identify α-glucosidase inhibitors from ginkgo biloba extract. As a result, four compounds were identified as α-glucosidase inhibitors in the ginkgo biloba extract, and their structures were confirmed to be quercetin, apigenin, kaempferol and isorhamnetin by ESI-MS.

Key words: Ginkgo biloba, α-Glucosidase inhibitor, Ultrafiltration mass spectrometry, Screening

CLC Number:

O657.63

TrendMD:

LIU Yang, ZHOU Hui, LIU Shu, LIU Zhi-Qiang. Screening of α-Glucosidase Inhibitors in Ginkgo Biloba Extract Using Ultrafiltration LC-ESI-MSⁿ[J]. Chem. J. Chinese Universities, 2013, 34(4): 813.

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Screening of α-Glucosidase Inhibitors in Ginkgo Biloba Extract Using Ultrafiltration LC-ESI-MSⁿ

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