Novel Nucleic Acid Signal Amplification Liquid Crystal Biosensor for Detection of p53 Gene Mutation

doi:10.7503/cjcu20121120

Abstract

Abstract:

A novel nucleic acid liquid crystal biosensor was constructed for the detection of the p53 mutant gene based on the branched nucleic acid hybridization reaction and the orientational transition of liquid crystal. Herein, a specific target DNA sequence was used as an initiator, the three different hairpin probes as dendritic monomers, and the branched-like DNA with controllable molecular size and weight could be formed by changing the concentrations of the monomers and the reaction time under mild conditions. And then the branched-like DNA was hybridized with the capture DNA sequences on the glass substrate. Making use of unique birefringent properties of liquid crystal molecules, the sensor could get an enhanced polarization response signal, and achieved the rapid and specific detection low to 1 nmol/L mutant DNA strands. This simple and sensitive detection method in the detection of DNA has greater practicality, and is expected to be promoted.

Key words: Hybridization chain reaction, Orientation transition, Branched-like DNA, Liquid crystal biosensor

CLC Number:

O657.39

TrendMD:

FAN Yu-Xia, TAN Hui, LIU Ling-Wei, WU Zhao-Yang, SHEN Guo-Li, YU Ru-Qin. Novel Nucleic Acid Signal Amplification Liquid Crystal Biosensor for Detection of p53 Gene Mutation[J]. Chem. J. Chinese Universities, 2013, 34(4): 806.

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