Amperometric Immunosensor Based on Layer-by-layer Assembly for Detecting PCB77 in Sludge Samples

doi:10.7503/cjcu20120869

Chem. J. Chinese Universities ›› 2013, Vol. 34 ›› Issue (5): 1092.doi: 10.7503/cjcu20120869

• Analytical Chemistry • Previous Articles Next Articles

Amperometric Immunosensor Based on Layer-by-layer Assembly for Detecting PCB77 in Sludge Samples

ZHANG Yan², ZHUANG Hui-Sheng¹, CHEN Han-Yu²

1. School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China;
2. College of Municipal and Environmental Engineering, Henan University of Urban Construction, Pingdingshan 467000, China

Received:2012-09-21 Online:2013-05-10 Published:2013-05-10
Contact: zhuang huisheng E-mail:huishengzhuang@126.com

Abstract

Abstract:

A new type of amperometric immunosensor for the determination of PCB77 was reported. Gold nanoparticles, glutaraldehyde and chitosan were assembled layer-by-layer into films on the gold electrode modified by L-cysteine for fabrication of the immunosensor. The preparation procedure of the sensor was characterized and the operation conditions, such as the pH value of supporting solution, incubated temperature and time were studied by differential pulse voltammetry. Under the optimized working conditions, the current response of the immunosensor was proportional to the concentration of naphthalene in the range of 0.1—160 ng/mL with a detection limit of 0.01 ng/mL(R=0.9964) . This method was applied to the PCB77 detection in sludge samples and the recovery was ranging in 95.0%—112.5%. The reported immunosensor exhibited high sensitivity and long-term stability for PCB77 detection, and was easy to be prepared and regenerated.

Key words: Layer-by-layer assembly, Label-free, Amperometric immunosensor, Gold nanoparticles, 3,3',4,4'-Tetrachlorobiphenyl(PCB77)

CLC Number:

O657

TrendMD:

ZHANG Yan, ZHUANG Hui-Sheng, CHEN Han-Yu. Amperometric Immunosensor Based on Layer-by-layer Assembly for Detecting PCB77 in Sludge Samples[J]. Chem. J. Chinese Universities, 2013, 34(5): 1092.

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Amperometric Immunosensor Based on Layer-by-layer Assembly for Detecting PCB77 in Sludge Samples

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