Scattering Imaging of Cervical Cancer Cell by Laser Scanning Confocal Microscopy with Gold Nano-probes

doi:10.3969/j.issn.0251-0790.2012.05.032

Chem. J. Chinese Universities ›› 2012, Vol. 33 ›› Issue (05): 1041.doi: 10.3969/j.issn.0251-0790.2012.05.032

• Physical Chemistry • Previous Articles Next Articles

Scattering Imaging of Cervical Cancer Cell by Laser Scanning Confocal Microscopy with Gold Nano-probes

CHEN Na¹, QIN Song-Bing², ZHANG Bing-Bo³, ZHANG Xu-Guang⁴, ZHOU Ju-Ying², TU Yu¹

1. School of Radiation Medicine and Protection, Soochow University, Suzhou 215123, China;
2. First Affiliated Hospital of Soochow University, Suzhou 215006, China;
3. Institute for Advanced Materials & Nano Biomedicine, Tongji University, Shanghai 200092, China;
4. Xuzhou Cancer Hospital, Xuzhou 221000, China

Received:2011-06-09 Online:2012-05-10 Published:2012-05-10

Abstract

Abstract: The quantities and activities of folate receptor are higher in tumor cells than those in normal cells, so it has become the ideal ligand for targeted therapy of tumor. Due to the unique optical property of gold nanoparticles(AuNPs), the scattering light from AuNPs can be detected when they are excited by a single laser. Based on these principles, in this study AuNPs with uniform size were prepared, then modified with folic acid(FA), the resulting AuNPs-FA were used as targeting probes for imaging of cervical cancer cells(Hela). The specific scattering imaging could be researched by laser scanning confocal microscopy(LSCM). The results show that the effect of intaking of AuNPs-FA is stronger than that of AuNPs by the cervical cancer cells. However, with the extension of time, the difference will gradually decrease. So the AuNPs-FA has a good targeting property to label the cervical cancer cells in the right time.

Key words: Gold nanoparticles, Folic acid, Laser scanning confocal microscopy(LSCM), Scattering imaging, Cervical cancer cell

CLC Number:

O648
Q503

TrendMD:

CHEN Na, QIN Song-Bing, ZHANG Bing-Bo, ZHANG Xu-Guang, ZHOU Ju-Ying, TU Yu. Scattering Imaging of Cervical Cancer Cell by Laser Scanning Confocal Microscopy with Gold Nano-probes[J]. Chem. J. Chinese Universities, 2012, 33(05): 1041.

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Scattering Imaging of Cervical Cancer Cell by Laser Scanning Confocal Microscopy with Gold Nano-probes

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