Vancomycin Derivative Modified Silica-coated Silver Nanoplate for Surface-enhanced Raman Scattering Imaging and Antimicrobial Photodynamic Therapy of Vancomycin Resistant Bacterial Strains†

doi:10.7503/cjcu20160758

Abstract

Abstract:

Silver nanoplates, as SERS-active cores, were encapsulated by silica shell and modified with porphyrin-vancomycin(Por-Van) molecules to prepare AgNPl@SiO₂-Por-Van composite. The Por-Van molecules conjugated on the AgNPl@SiO₂ core/shell nanostructure were applied for surface enhanced raman scattering(SERS) detection and antimicrobial photodynamic therapy(aPDT) of Vancomycin resistant enterococci(VRE) strains. Results for in vitro bacterial SERS imaging revealed that the core/shell nanostructure highly enhanced specific binding affinity towards VRE bacteria. Meanwhile, excellent bactericidal effect was also obtained through in vitro and in vivo photodynamic therapy studies. Therefore, the AgNPl@SiO₂-Por-Van indicated high possibility for applications in theranostics towards VRE bacteria, integrating SERS imaging and aPDT.

Key words: Silver nanoplates(AgNPls), Surface-enhanced Raman scattering imaging, Antimicrobial photodynamic therapy(aPDT), Vancomycin resistant enterococci(VRE)

CLC Number:

O644

TrendMD:

JIANG Tingting, WANG Yuanfang, LIU Peilong, TIAN Yixia, LI Hao, HU Yongguo. Vancomycin Derivative Modified Silica-coated Silver Nanoplate for Surface-enhanced Raman Scattering Imaging and Antimicrobial Photodynamic Therapy of Vancomycin Resistant Bacterial Strains^†[J]. Chem. J. Chinese Universities, 2017, 38(5): 846.

Figures/Tables 11

Fig.1 Synthesis of AgNPl@SiO2-Por-Van

Fig.2 Structure and synthetic route of Por-Van

Fig.3 TEM images of AgNPls(A) and AgNPl@SiO2(B) and dynamic light scattering result of AgNPl@SiO2-Por-Van(C)

Fig.4 Raman spectra(A) and UV-Vis specta(B, C) of AgNPl@SiO2-Por-Van and relative moieties(C)

Fig.5 FTIR spectra of the Por-Van(a), AgNPl@SiO2(b) and AgNPl@SiO2-Por-Van(c)

Table 1 Minimum inhibition concentration(MIC) of AgNPl@SiO2-Por-Van and relative moieties towards Vancomycin sensitive and resistant bacterial strains

Sample	MIC/(μmol·L^-1)
Sample	B. Subtilis	E. Faecium	E. Faecalis
Van	1	>88	44
Por-Van	3	>15^*	>15^*
AgNPl@SiO₂-Por-Van	0.005	>0.010^*	>0.010^*

Fig.6 SERS(A1—C1) and the original Raman spectra(A2—C2) of five different points(A1)—(C1) Merged pictures of photographic images and Raman mappings of different bacterial strains. The maps are constructed on the band area at 1075 cm-1; (A2)—(C2) the Raman spectra in colors are collected from the points highlighted with same color by Raman confocal microscopy. (A1, A2) B. Subtilis; (B1, B2) E. Faecium(Van A); (C1, C2) E. Faecalis(Van B).

Fig.7 Dose(A, B) and light(C, D) dependent photodynamic inactivation effect of Por, Por-Van and AgNPl@SiO2-Por-Van towards E. faecium(VanA)(A, C) and E. faecalis(VanB)(B, D) (A, B) The irradiation time is 30 min; (B, D) the concentration is 10 nmol/L.

Fig.8 MTT assays for cytotoxicity and phototoxicity of AgNPl@SiO2-Por-Van Illumination time: 30 min.

Fig.9 Color photographs of infection sites(wounds created at the mice back) of animals from different experimental groups(A1)—(A5) Non-treated; (B1—B5) AgNPl@SiO2-Por-Van treated without illumination; (C1)—(C5) AgNPl@SiO2-Por-Van treated with illumination. Time/d: (A1—C1) 0; (A2—C2) 1; (A3—C3) 2; (A4—C4) 3; (A5—C5) 4.

Fig.10 Quantitification of bacterial infection after the in vivo aPDT treatment at the fourth daya. Non-treated; b. AgNPl@SiO2-Por-Van treated without illumination; c. AgNPl@SiO2-Por-Van treated with illumination.

References 28

[1]	Xing B., Jiang T., Bi W., Yang Y., Li L., Ma M., Chang C. K., Xu B., Yeow E. K., Chem. Commun., 2011, 47(5), 1601—1603
[2]	Patel R., Gallagher J. C., Ann. Pharmacother., 2015, 49(1), 69—85
[3]	Putty S., Vemula H., Bobba S., Gutheil W. G., Anal. Biochem., 2013, 442(2), 166—171
[4]	Li L., Xu B., Curr. Pharm. Des., 2005, 11(24), 3111—3124
[5]	Gao Y., Qiao G., Zhuo L., Li N., Liu Y., Tang B., Chem. Commun., 2011, 47(18), 5316—5318
[6]	Jiang C., Cheng H., Yuan A., Tang X., Wu J., Hu Y., Acta Biomater., 2015, 14(1), 61—69
[7]	Yuan A., Yang B., Wu J., Hu Y., Ming X., Acta Biomater., 2015, 21(1), 63—73
[8]	Shi J., Chen Z., Wang L., Wang B., Xu L., Hou L., Zhang Z., Acta Biomater., 2016, 29(1), 282—297
[9]	Vatansever F., de Melo W. C., Avci P., Vecchio D., Sadasivam M., Gupta A., Chandran R., Karimi M., Parizotto N. A., Yin R., Tegos G. P., Hamblin M. R., FEMS Microbiol. Rev., 2013, 37(6), 955—989
[10]	Al-Ahmad A., Tennert C., Karygianni L., Wrbas K. T., Hellwig E., Altenburger M. J., J. Med. Chem., 2013, 62(3), 467—473
[11]	Niu X., Chen H., Wang Y., Wang W., Sun X., Chen L., ACS Appl. Mater. Interfaces, 2014, 6(7), 5152—5160
[12]	Fang H., Zhang C. X., Liu L., Zhao Y. M., Xu H. J., Biosens. Bioelectron., 2015, 64(2), 434—441
[13]	Tan Y. B., Zou J. M., Gu N., Nanoscale Res. Lett., 2015, 10(1), 417—424
[14]	Zhao L., Kim T. H., Kim H. W., Ahn J. C., Kim S. Y., Acta Biomater., 2015, 20(1), 155—164
[15]	Wang Y., Yan B., Chen L., Chem. Rev., 2013, 113(3), 1391—1428
[16]	Lin D., Qin T., Wang Y., Sun X., Chen L., ACS Appl. Mater. Interfaces, 2014, 6(2), 1320—1329
[17]	Zhang W., Wang Y., Sun X., Wang W., Chen L., Nanoscale, 2014, 6(23), 14514—14522
[18]	Wang Y., Chen L., Liu P., Chemistry, 2012, 18(19), 5935—5943
[19]	Zhang J. L., Ma X., Xu M. X., Zong Y., Ren B., Chem. J. Chinese Universities, 2016, 37(7), 1257—1261
	(张金亮, 马鑫, 徐梦溪, 宗铖, 任斌.高等学校化学学报, 2016,37(7), 1257—1261)
[20]	Zou X., Dong S., J. Phys. Chem. B, 2006, 110(43), 21545—21550
[21]	Gao C., Lu Z., Liu Y., Zhang Q., Chi M. I., Cheng Q., Yin Y., Angew. Chem. Int. Ed. Engl., 2012, 51(23), 5629—5633
[22]	Xia Y., Ye J., Tan K., Wang J., Yang G., Anal. Chem., 2013, 85(13), 6241—6247
[23]	Ung T., Liz-Marzan L. M., Mulvaney P., Langmuir, 1998, 14(14), 3740—3748
[24]	Liz-Marzan L. M., Giersig M., Mulvaney P., Langmuir, 1996, 12(18), 4329—4335
[25]	Murphy C.J., Gole A. M., Hunyadi S. E., Stone J. W., Sisco P. N., Alkilany A., Kinard B. E., Hankins P.,Chem. Commun., 2008, (5), 544—557
[26]	Prevo B. G., Esakoff S. A., Mikhailovsky A., Zasadzinski J. A., Small, 2008, 4(8), 1183—1195
[27]	Xia L., Kim N. H., Kim K., J. Colloid Interface Sci., 2007, 306(1), 50—55
[28]	González B., Colilla M., Vallet-Regí M., Chem. Mater., 2008, 20(15), 4826—4834