高等学校化学学报

高等学校化学学报

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大鼠骨髓间充质干细胞分化过程的比较蛋白质组学研究

叶能胜, 张荣利, 罗国安, 张会亮, 赵艳峰, 张敏, 王义明   

  1. 清华大学生命科学与医学研究院, 北京 100084
  • 收稿日期:2005-09-19 修回日期:1900-01-01 出版日期:2006-10-10 发布日期:2006-10-10
  • 通讯作者: 罗国安

Comparative Proteomics Analysis of Rat Mesenchymal Stem Cells During Induced Differentiation

YE Neng-Sheng, ZHANG Rong-Li, LUO Guo-An, ZHANG Hui-Liang, ZHAO Yan-Feng, ZHANG Min, WANG Yi-Ming   

  1. Institute of Life Science and Biomedicine, Tsinghua University, Beijing 100084, China
  • Received:2005-09-19 Revised:1900-01-01 Online:2006-10-10 Published:2006-10-10
  • Contact: LUO Guo-An

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被引次数

10

摘要： 从蛋白质组学角度分析大鼠骨髓间充质干细胞(MSCs)体外定向分化为心肌细胞过程中蛋白表达情况, 采用二维电泳分离蛋白, 用PDQuest软件分析蛋白表达差异, 并采用质谱(MALDI-TOF-MS)进行鉴定, 得到了54个蛋白点, 对蛋白的生物功能分析表明, 部分蛋白通过不同的信号途径参与了MSCs的分化过程.

关键词: 骨髓间充质干细胞, 蛋白质组学, 分化, 机理

Abstract: Bone marrow mesenchymal stem cells(MSCs) can be differentiated into cardiomyocytes with the induction of 5-azacytidine in vitro, and the induced cardiomyocytes can be used for cell transplantation for cardiac diseases. In the present study, the protein profiling of rat MSCs was investigated by two-dimensional gel electrophoresis(2DE). According to the data analysis by PDQuest, 24 protein spots showed up-regulation, and 14 protein spots showed down-regulation during MSCs differentiation. However, there were 9 protein spots expressed only in undifferentiated MSCs, and 19 protein spots were detected in differentiated cells but not MSCs. Altered proteins were digested by trypsin and subjected to MALDI-TOF-MS analysis for peptide mass fingerprinting(PMF). Fifty-four proteins were identified successfully from NCBInr and/or SWISS-PROT databases. Among the identified proteins, there were 20 proteins showed up-regulation, and 4 proteins down-regulated during the induced differentiation of MSCs. According to the previous reports of their function, these regulated proteins may be involved in the differentiation of rat MSCs. Our data suggested that proteomics can be used to highlight the study of molecular mechanism of cell differentiation.

Key words: Bone marrow mesenchymal stem cells(MSC), Proteomics, Differentiation, Mechanism

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