高等学校化学学报

高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (12): 2238.

• 研究论文 • 上一篇    下一篇

基于Au纳米通道膜的生物分离新方法在蛋白质分离中的应用

黄杉生, 殷月芬, 王柯敏, 何晓晓, 钟桐生   

  1. 湖南大学化学生物传感与计量学国家重点实验室, 化学化工学院, 湖南省生物纳米工程技术研究中心, 长沙410082
  • 收稿日期:2003-10-08 出版日期:2004-12-24 发布日期:2004-12-24
  • 基金资助:

    国家自然科学基金重点项目(批准号:20135010);国家重点基础研究发展规划项目(批准号:2002CB513100-10);国家科技攻关计划项目(批准号:2003BA3310A16);科技部国际合作重点项目(批准号:2003DF000039)资助

A New Biochemical Method for Protein Separation Based on Gold Nanotubule Membrane

HUANG Shan-Sheng, YIN Yue-Fen, WANG Ke-Min, HE Xiao-Xiao, ZHONG Tong-Sheng   

  1. State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry & Chemical Engineering, Engineering Research Center for Bio-Nanotechnology of Hunan Province, Hunan University, Changsha 410082, China
  • Received:2003-10-08 Online:2004-12-24 Published:2004-12-24

PDF (PC)

被引次数

34

摘要： 采用化学沉积法将Au沉积到聚碳酸酯滤膜(PC-Mem)上,制备了直径为55nm左右的Au纳米通道膜(Au-Mem).以牛血清白蛋白(BSA)和免疫球蛋白G(IgG)抗体为模型分子,研究了Au-Mem以及分别以半胱氨酸和硫氰酸胍修饰的Au-Mem(Cys-Au-Mem,Gua-Au-Mem)对模型分子的分离特性.由于硫氰酸胍具有一定的亲水作用和蛋白质变性作用,BSA在Gua-Au-Mem上的迁移速率比在Au-Mem上增加了30～50倍,而修饰膜对IgG的迁移速率无明显影响.在pH=4.5时分离了BSA和IgG的混合溶液.Gua-Au-Mem和Cys-Au-Mem对混合溶液的分离度分别为31.6和23.1,表明经修饰的Au-Mem具有良好的分离性能.

关键词: Au纳米通道膜, 蛋白质, 迁移, 分离

Abstract: Gold nanotubule membranes with about 55 nm of diameter were prepared by chemical deposition of gold on polycarbonate templates membrane. After being modified by cysteine and guanide thiocyanate, the nanomembranes were studied with BSA and IgG as the model molecules. The results show that guanide thiocyanate facilitated transporting of BSA by 30 to 50 times because of hydrophilic and denaturalization effects whereas IgG almost retained its′ transporting speed. Then the separation processes were carried out and separation degrees of 31.6 and 23.1 were obtained for cysteine and guanide thiocyanate modified gold nanotubules, respectively at pH 4.5. The results indicate that gold nanotubule membranes has a good separating capability for protein.

Key words: Gold nanotubule membrane, Protein, Transporting, Separation

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