高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    

Fe-N共掺杂碳量子点作为高活性类过氧化物酶用于绿原酸比色检测

张敏1，张文皓2，李光英1，王丽敏3，单桂晔3   

  1. 1. 长春中医药大学附属医院 2. 青岛海关技术中心 3. 东北师范大学物理学院
  • 收稿日期:2025-10-10 修回日期:2025-11-09 网络首发:2025-11-13 发布日期:2025-11-13
  • 通讯作者: 李光英 E-mail:shangy229@nenu.edu.cn
  • 基金资助:
    国家自然科学基金面上项目（1257041767）；吉林省自然科学基金（批准号：20200201582JC ）资助

Fe-N co-doped carbon quantum dots as highly active peroxidase mimics for colorimetric detection of chlorogenic acid

ZHANG Min1, ZHANG Wenhao2, LI Guangying1, WANG Limin3, SHAN Guiye3   

  1. 1. the Affiliated Hospital of Changchun University of Chinese Medicine

    2. Technology Center of Qingdao Customs 3. School of Physics, Northeast Normal University

  • Received:2025-10-10 Revised:2025-11-09 Online First:2025-11-13 Published:2025-11-13
  • Contact: Guang-Ying LI E-mail:shangy229@nenu.edu.cn
  • Supported by:
    Supported by the Department of Science and Technology of JiLin Province (No. 20200201582JC )

PDF (PC)

摘要： 以L-组氨酸（His）和七水合硫酸亚铁（FeSO?·7H?O）为前驱体，通过一步水热法成功制备了铁、氮共掺杂碳量子点（Fe-N-CDs）。Fe原子的引入在碳量子点表面引入了更多活性位点，促进了催化过程中的电子转移，从而提升了材料的催化效率。所制备的Fe-N-CDs可催化过氧化氢（H2O2）分解产生羟基自由基（?OH），进而氧化显色剂TMB（3,3’,5,5’-四甲基联苯胺）生成蓝色的氧化产物oxTMB，该产物在652 nm处具有明显的紫外-可见吸收峰。绿原酸（CGA）作为一种天然多酚类化合物，广泛存在于植物中，具有良好的抗氧化性能。CGA能够将氧化态的oxTMB还原为无色的TMB，导致体系蓝色褪去，吸收峰强度降低。基于这一现象，可通过吸光度的变化构建CGA含量与吸光强度之间的定量关系，实现对食品中绿原酸含量的快速检测。实验结果表明，该方法具有良好的选择性、抗干扰能力与快速响应特性，能够对食品中的抗氧化成分实现可视化检测，为食品质量评估提供了一种有效手段。

关键词: 碳点, 纳米酶, 绿原酸, 比色检测

Abstract: Fe, N-doped carbon quantum dots (Fe-N-CDs) were synthesized via a one-step hydrothermal method using L-histidine (His) and ferrous sulfate heptahydrate (FeSO?·7H?O) as precursors. The introduction of Fe atoms may increase the number of active sites on the N-CDs surface and facilitate electron transfer during catalysis, thereby enhancing catalytic reaction rates and efficiency. The Fe-N-CDs exhibited significantly enhanced peroxidase-like activity. In the catalytic reaction, Fe-N-CDs decomposed H?O? to generate hydroxyl radicals (?OH), which oxidized colorless TMB (3,3',5,5'-tetramethylbenzidine) to blue oxidized TMB (oxTMB), producing a distinct UV-Vis absorption peak at 652 nm. Chlorogenic acid (CGA), known for its unique antioxidant properties, was introduced into this chromogenic system. The characteristic absorption peak of oxTMB disappeared, accompanied by gradual fading of the blue color. This phenomenon enables the detection of CGA in food samples. This study not only developed a carbon-based nanozyme with superior catalytic activity but also established a simple, rapid, and highly sensitive method for CGA detection.

Key words: Carbon dots, Naoeezymes, Chlorogenic acid, Colorimetric detection

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