高等学校化学学报

高等学校化学学报

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[碳点：合成、结构与功能]基于细胞衍生荧光碳点的葡萄糖比色及荧光测定

刘瀛琪1,王叶梅1,蒋恺1,郑芬芬1,朱俊杰2   

  1. 1. 江苏科技大学
    2. 南京大学
  • 收稿日期:2024-08-15 修回日期:2024-09-19 出版日期:2024-09-20 发布日期:2024-09-20
  • 通讯作者: 朱俊杰
  • 基金资助:
    中国国家自然科学基金(批准号：21804059)和江苏省研究生科研与实践创新计划项目(批准号：KYCX23_3898)资助

Colorimetric and fluorescence determination of glucose based on cell-derived fluorescent carbon dots

LIU Yingqi1, WANG Yemei1, JIANG Kai1, ZHENG Fenfen1, ZHU Jun-Jie2   

  1. 1. Jiangsu University of Science and Technology
    2. Nanjing University
  • Received:2024-08-15 Revised:2024-09-19 Online:2024-09-20 Published:2024-09-20
  • Contact: Zhu Junjie
  • Supported by:
    Supported by the National Natural Science Foundation of China (No.21804059) and the Postgraduate Research & Practice Innovation Program of Jiangsu Province (No. KYCX23_3898)

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摘要： 以肿瘤细胞为原料，通过一步水热法合成了细胞衍生荧光碳点(CDs). 研究发现，该CDs具有优异的类过氧化物酶催化活性，能够催化过氧化氢反应生成羟基自由基. 基于此，利用葡萄糖氧化产生的过氧化氢以及CDs的类过氧化物酶性能氧化对苯二胺(p-phenylenediamine, PPD)底物生成氧化产物PPDox (2,5-diamino-N,N’-di-(4-aminophenyl)-2,5-cyclohexadiene-1,4-diimine). 并通过PPDox对荧光CDs的内滤效应，实现葡萄糖的比色与荧光双模检测. 比色法与荧光法检测葡萄糖的检测限分别为41μmol/L和13μmol/L. 本方法中，CDs具备双重功能，既可以作为类过氧化物酶催化过氧化氢产生羟基自由基，用于氧化PPD，还可以作为荧光指示剂，通过内滤效应，指示葡萄糖的浓度变化.

关键词: 细胞衍生荧光碳点, 类过氧化物酶, 葡萄糖探针

Abstract: Cell derived fluorescent carbon points (CDs) were synthesized via one step hydrothermal method. It is found that the CDs have excellent peroxide-like catalytic activity, which can catalyze the hydrogen peroxide to produce hydroxyl radical. Based on this, the hydrogen peroxide produced by glucose oxidation and CDs could consecutively oxidize p-phenylenediamine (PPD to produce PPDox (2,5-Diamino-N,N'-di-(4-aminophenyl)-2,5-cyclohexadiene-1,4-diimine). By virtue of the inner filter effect of PPDox on CDs, a dual-mode colorimetric and fluorescent detection of glucose was establised. The limit of detection of the present assay is 41 μmol/L and 13 μmol/L for the colorimetric method and the fluorescence method, respectively. In this work, CDs can not only be used as a peroxide-like enzyme to catalyze hydrogen peroxide to produce hydroxyl radical for PPD oxidation, but also be used as a fluorescent indicator to indicate the change of glucose concentration through the internal filtration effect.

Key words: Cell derived fluorescent carbon dots, Peroxide-like, Glucose probe

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