高等学校化学学报 ›› 2020, Vol. 41 ›› Issue (1): 102-110.doi: 10.7503/cjcu20190385

• 分析化学 • 上一篇    下一篇

以DNA为模板的银纳米簇荧光检测及逻辑门的构建

肖周锐,黄东华,边孟孟,袁亚利(),聂瑾芳   

  1. 桂林理工大学化学与生物工程学院, 桂林 541004
  • 收稿日期:2019-07-10 出版日期:2019-12-17 发布日期:2019-10-29
  • 通讯作者: 袁亚利 E-mail:thanksin2013@163.com
  • 基金资助:
    国家自然科学基金批准号:(21765005);广西自然科学基金批准号:(2018GXNSFAA138047);广西科技计划项目批准号: 桂科(AD19110096);广西中青年教师基础能力提升项目资助批准号:(2018KY0260)

Fluorescence Detection Based on DNA-templated Silver Nanoclusters and the Construction of Multi-level Logic Gate

XIAO Zhourui,HUANG Donghua,BIAN Mengmeng,YUAN Yali(),NIE Jinfang   

  1. College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China
  • Received:2019-07-10 Online:2019-12-17 Published:2019-10-29
  • Contact: Yali YUAN E-mail:thanksin2013@163.com
  • Supported by:
    ? Supported by the National Natural Science Foundation of China No(21765005);the Guangxi Natural Science Foundation, China No(2018GXNSFAA138047);the Science and Technology Project of Guangxi, China No.GUIKE(AD19110096);the Foundation of Guangxi Education Office No(2018KY0260)

摘要:

以DNA为模板, 合成了具有荧光性质的银纳米簇(DNA-Ag NCs), 利用荧光光谱、 紫外光谱和红外光谱等手段对其进行了表征. 基于DNA-Ag NCs与离子相互作用时产生的荧光变化可实现对离子浓度的检测. 实验结果表明, 在最佳实验条件下, Ni 2+及Hg 2+的浓度与DNA-Ag NCs荧光强度呈线性关系; 并验证了该荧光探针用于检测自来水样品中汞离子和镍离子的实用性. 由于以DNA为模板的DNA-Ag NCs能够响应多种刺激, 如Ni 2+, S 2-, Hg 2+和pH等, 利用相应的荧光强度可构建多输入的DNA-Ag NCs逻辑门及其组合逻辑门. 当荧光输出强度(Ioutput)>初始荧光强度(Iorigin)时, 设定输出为1, 采用各种刺激及其组合作为输入, 构建了YES, INH和组合的NOR与INH逻辑门. 而只有当IoutputIorigin时定义为输出为1, 可建立NOT, NOR, 组合的IMP加上NOR与AND逻辑门. 基于DNA-Ag NCs可以构建响应多元输入的复杂逻辑门, 实现化学信息的转变和传输, 在构建新的分子器件方面有较大应用前景.

关键词: 荧光检测, DNA模板, 银纳米簇, 逻辑门

Abstract:

Fluorescent silver nanoclusters were synthesized using DNA as template(DNA-Ag NCs), and characterized by fluorescence, ultraviolet and infrared spectroscopy. According to the fluorescence changes generated by the interaction between DNA-Ag NCs and ions, relative concentration detections can be carried out. Under the optimal experimental conditions, the concentrations of Ni 2+ and Hg 2+ showed a linear relationship with the fluorescence intensity of DNA-Ag NCs. The practicability of this fluorescent probe in tap water samples is also verified. Since DNA-Ag NCs can respond to a variety of stimuli, including Ni 2+, S 2-, Hg 2+ and H +(pH=7.0), logic gates of multiple input and their combination can be constructed based on the corresponding fluorescence intensity. When definition of fluorescence output intensity(Ioutput)>initial fluorescence intensity(Iorigin) as output=1 was adopted, the YES, INH and integrative NOR and INH logic gates were constructed. And if IoutputIorigin is defined as output=1, the NOT, NOR and integrative IMP, NOR and AND logic gates can be established. Based on DNA-Ag NCs, complex logic gates involving multiple inputs can be constructed to realize the transformation and transmission of chemical information, which has great application prospects in constructing new molecular devices.

Key words: Fluorescence detection, DNA template, Silver nanoclusters, Logic gate

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