苦参碱和槐定碱在多刺激响应性水凝胶薄膜电极上的可调控电化学行为及其逻辑门构建

doi:10.7503/cjcu20180741

高等学校化学学报 ›› 2019, Vol. 40 ›› Issue (4): 685.doi: 10.7503/cjcu20180741

苦参碱和槐定碱在多刺激响应性水凝胶薄膜电极上的可调控电化学行为及其逻辑门构建

吴雪梅¹, 韦洁¹, 许红平², 左玲霞², 单晓艳², 姚惠琴^1,²(), 郑志祥³

1. 宁夏医科大学药学院
2. 基础医学院, 银川 750004
3. 甘肃政法学院, 甘肃省证据科学技术研究与应用重点实验室, 兰州 730070

收稿日期:2018-11-01 出版日期:2019-04-03 发布日期:2019-01-09
作者简介:
联系人简介: 姚惠琴, 女, 博士, 教授, 主要从事生物/药物电分析化学与逻辑门网络方面的研究. E-mail: huiqin_yao@163.com
基金资助:
宁夏自然科学基金(批准号: NZ17052)、大学生创新实验项目(批准号: NXCX2016139)、国家自然科学基金(批准号: 21665021)和西部一流基础医学学科项目资助.

Switchable Electrochemical Behavior of Matrine and Sophoridine at Multi-stimuli Responsive Hydrogel Film Electrode and Construction of Logic Gate^†

WU Xuemei¹, WEI Jie¹, XU Hongping², ZUO Lingxia², SHAN Xiaoyan², YAO Huiqin^1,^2,^*(), ZHENG Zhixiang³

1. College of Pharmacy, 2. School of Basic Medical Sciences, Ningxia Medical University, Yinchuan 750004, China
2. School of Basic Medical Sciences, Ningxia Medical University, Yinchuan 750004, China
3. Key Laboratory of Evidence Science Technology Research and Application, Gansu Institute of Political Science and Law, Lanzhou 730070, China

Received:2018-11-01 Online:2019-04-03 Published:2019-01-09
Contact: YAO Huiqin E-mail:huiqin_yao@163.com
Supported by:
† Supported by the Natural Science Foundation of Ningxia, China(No.NZ17052), the College Students' Innovation Experiment of Ningxia, China(No.NXCX2016139), the National Natural Science Foundation of China(No. 21665021) and the West China Top Class Discipline Project in Basic Medical Sciences.

摘要/Abstract

摘要：

采用苦参碱(Matrine, MT)和槐定碱(Sophoridine, SR)作为电活性药物分子探针, 考察了聚(N-异丙基)丙烯酰胺(PNIPAM)水凝胶薄膜的刺激响应特性; 并通过循环伏安(CV)法和扫描电子显微镜(SEM)研究了PNIPAM在不同外界刺激条件(如温度、盐浓度和甲醇)下结构的改变. 在25 ℃时, 药物分子MT和SR在玻碳电极上产生较大的氧化峰电流, 随着温度升高至40 ℃, 药物分子探针的氧化峰电流逐渐减小. 考察了不同Na₂SO₄浓度和不同比例甲醇对PNIPAM水凝胶薄膜的影响, 发现MT和SR的氧化峰电流随着Na₂SO₄浓度(00.45 mol/L)和甲醇比例(040%)的升高而减小. 结果表明, MT和SR可作为分子探针研究PNIPAM水凝胶薄膜对环境的三重刺激响应性开关行为, 并可进一步构筑3-输入/2-输出的逻辑门系统.

关键词: 苦参碱, 槐定碱, 聚(N-异丙基)丙烯酰胺, 可调控, 逻辑门

Abstract:

Matrine(MT) and sophoridine(SR) were used as electroactive drug molecular probes to investigate the stimuli-responsive characteristics of the hydrogels poly(N-isopropyl)acrylamide(PNIPAM), and the changes of PNIPAM structure under different external stimuli(temperature, salt concentration and methanol) were observed by cyclic voltammetry(CV) method and scanning electron microscopy(SEM). The results show that at 25 ℃, MT and SR lead to a large oxidation peak current; when the temperature reached at 40 ℃, the oxidation peak currents of MT and SR are gradually reduced. The influence of different Na₂SO₄ concentration and different proportion of methanol on PNIPAM hydrogel were also investigated. The oxidation peak currents of MT and SR decreased with the increasing of Na₂SO₄ concentration(0—0.45 mol/L) and volume fraction of methanol ratio(0—40%). The results indicate that MT and SR could be used as molecular probes to investigate the switching behaviors of PNIPAM hydrogel films, and further constructed 3-input/2-output logic gate system.

Key words: Matrine, Sophoridine, Poly(N-isopropyl)acrylamide, Controllable, Logic gate

TrendMD:

吴雪梅, 韦洁, 许红平, 左玲霞, 单晓艳, 姚惠琴, 郑志祥. 苦参碱和槐定碱在多刺激响应性水凝胶薄膜电极上的可调控电化学行为及其逻辑门构建. 高等学校化学学报, 2019, 40(4): 685.

WU Xuemei,WEI Jie,XU Hongping,ZUO Lingxia,SHAN Xiaoyan,YAO Huiqin,ZHENG Zhixiang. Switchable Electrochemical Behavior of Matrine and Sophoridine at Multi-stimuli Responsive Hydrogel Film Electrode and Construction of Logic Gate^†. Chem. J. Chinese Universities, 2019, 40(4): 685.

图/表 9

Scheme 1 Chemical structures of MT(A) and SR(B)

Fig.1 CVs of 0.01 mol/L MT(A) and SR(B) at different pH values and effects of pH value on the Ipa and oxidation peak potential(Epa) of MT(C) and SR(D)pH: a. 4.0; b. 5.0; c. 6.0; d. 7.0; e. 8.0; f. 9.0.

Fig.2 FTIR spectra of PNIPAM(a) and NIPAM(b) samples

Fig.3 CVs curves of 0.01 mol/L SR at 0.05 V/s in pH=9.0 NaCl solution without Na2SO4 for PNIPAM films at different temperatures(A), influence of solution temperature on Ipa of SR(a) and MT(b) in pH=9.0 NaCl solution(B) and variation of Ipa of 0.01 mol/L SR at 0.05 V/s in pH=9.0 NaCl solution with immersion time at temperature switched between 25 ℃ and 40 ℃ for the same PNIPAM films(C)(A) Temperature/℃: a. 25; b. 27; c. 29; d. 31; e. 33; f. 35; g. 37; h. 39; i. 40.

Fig.4 SEM top-view images of PNIPAM hydrogel films on GC surface after treated with pH=9.0 NaCl solutions(A) At 25 ℃; (B) at 40 ℃; (C) containing 0.45 mol/L Na2SO4 at 25 ℃; (D) containing 20%(volume fraction) methanol at 25 ℃.

Fig.5 CVs curves of 0.01 mol/L MT at 0.05 V/s in pH=9.0 NaCl solutions containing 0(a), 0.05(b), 0.10(c), 0.15(d), 0.20(e), 0.25(f), 0.30(g), 0.35(h), 0.40(i) and 0.45 mol/L(j) Na2SO4 for PNIPAM films at 25 ℃(A), variation of Ipa of 0.01 mol/L MT with immersion time containing 0 and 0.45 mol/L Na2SO4(B) and dependence of Ipa of 0.01 mol/L MT at 0.05 V/s in pH=9.0 NaCl solutions at 25 ℃ on Na2SO4(a), MgSO4(b), NaCl(c) and NaNO3(d) for PNIPAM films(C)

Fig.6 Influence of methanol volume fraction(0—40%) in water/methanol mixture solvent on Ipa of MT in pH=9.0 solutions for PNIPAM films at 25 ℃(A), variation of Ipa with immersion time in solutions containing 0() and 20%() methanol(B) and CVs of 0.01 mol/L MT containing 0(a) and 20%(b) methanol at bare GC electrodes(scan rate: 0.05 V/s)(C)

Fig.7 Ipa(CVs) obtained after application of eight combinations of the three input signalsThe dashed lines show the threshold values separating output signals. Inset: logic circuit for the 3-input/2-output logic gate system(input A=temperature, input B=Na2SO4, input C=methanol).

Table 1 Truth values of 3-input/2-output logic gate system

Input A(Temperature)	1	1	1	0	0	0
Input B(S $O 4 2 -$ )	1	1	0	1	1	0
Input C(Methanol)	1	0	1	1	0	1
Output(I_pa)	1	0	0	0	0	0

Table 1 Truth values of 3-input/2-output logic gate system

Input A(Temperature)	1	1	1	0	0	0
Input B(S $O 4 2 -$ )	1	1	0	1	1	0
Input C(Methanol)	1	0	1	1	0	1
Output(I_pa)	1	0	0	0	0	0

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苦参碱和槐定碱在多刺激响应性水凝胶薄膜电极上的可调控电化学行为及其逻辑门构建

Switchable Electrochemical Behavior of Matrine and Sophoridine at Multi-stimuli Responsive Hydrogel Film Electrode and Construction of Logic Gate^†

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苦参碱和槐定碱在多刺激响应性水凝胶薄膜电极上的可调控电化学行为及其逻辑门构建

Switchable Electrochemical Behavior of Matrine and Sophoridine at Multi-stimuli Responsive Hydrogel Film Electrode and Construction of Logic Gate†

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Switchable Electrochemical Behavior of Matrine and Sophoridine at Multi-stimuli Responsive Hydrogel Film Electrode and Construction of Logic Gate^†